High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins

Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F12%3A00377502" target="_blank" >RIV/61388971:_____/12:00377502 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1007/s10858-012-9613-x" target="_blank" >http://dx.doi.org/10.1007/s10858-012-9613-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10858-012-9613-x" target="_blank" >10.1007/s10858-012-9613-x</a>

Jazyk výsledku
angličtina
Název v původním jazyce
High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins
Popis výsledku v původním jazyce
Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using delta subunit (20 kDa) of Bacillus subtilis RNA polymerase that has an 81-amino acid disordered part containing various repetitive sequences
Název v anglickém jazyce
High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins
Popis výsledku anglicky
Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using delta subunit (20 kDa) of Bacillus subtilis RNA polymerase that has an 81-amino acid disordered part containing various repetitive sequences

Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
—

Projekt
<a href="/cs/project/GA204%2F09%2F0583" target="_blank" >GA204/09/0583: Delta podjednotka RNA polymerázy z Gram pozitivních bakterií</a><br>
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Podobné výsledky(10)