Identification of cytokinin receptors of Brassica napus
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F12%3A00064588" target="_blank" >RIV/00216224:14740/12:00064588 - isvavai.cz</a>
Výsledek na webu
<a href="https://vufind.mzk.cz/Record/MZK01-001228453/UserComments" target="_blank" >https://vufind.mzk.cz/Record/MZK01-001228453/UserComments</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Identification of cytokinin receptors of Brassica napus
Popis výsledku v původním jazyce
Cytokinins (CK) belong to a group of plant hormones, playing crucial role in plant growth and development. CK signal is transduced through a multistep phosphorelay where the phosphoryl group is transferred between His and Asp residues. Cytokinin perception has been well-studied and the CHASE-containing sensor His kinases (CHKs) have been shown to be important morphological factors in Arabidopsis. As Brassica napus shares a high degree of nucleotide identity in coding regions with Arabidopsis, we used sequences of A. thaliana His kinase genes (AHKs) to search for their orthologs in B. napus (BnCHKs). Experimental screen of bacterial artificial chromosome (BAC) genomic DNA library of B. napus, var. Tapidor (JBnB), resulted in identification of redundantnumber of overlapping BAC clones, which hybridized with PCR-generated probes derived from sequences of phylogenetically conserved AHK regions. Five selected clones have been sequenced and defined as CK receptors by computational analysis.
Název v anglickém jazyce
Identification of cytokinin receptors of Brassica napus
Popis výsledku anglicky
Cytokinins (CK) belong to a group of plant hormones, playing crucial role in plant growth and development. CK signal is transduced through a multistep phosphorelay where the phosphoryl group is transferred between His and Asp residues. Cytokinin perception has been well-studied and the CHASE-containing sensor His kinases (CHKs) have been shown to be important morphological factors in Arabidopsis. As Brassica napus shares a high degree of nucleotide identity in coding regions with Arabidopsis, we used sequences of A. thaliana His kinase genes (AHKs) to search for their orthologs in B. napus (BnCHKs). Experimental screen of bacterial artificial chromosome (BAC) genomic DNA library of B. napus, var. Tapidor (JBnB), resulted in identification of redundantnumber of overlapping BAC clones, which hybridized with PCR-generated probes derived from sequences of phylogenetically conserved AHK regions. Five selected clones have been sequenced and defined as CK receptors by computational analysis.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/LC06034" target="_blank" >LC06034: Regulace morfogeneze rostlinných buněk a orgánů</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů