Toward positional cloning of Fhb1, a major QTL for Fusarium head blight resistance in wheat

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F08%3A00337704" target="_blank" >RIV/61389030:_____/08:00337704 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Toward positional cloning of Fhb1, a major QTL for Fusarium head blight resistance in wheat

Popis výsledku v původním jazyce

DNA markers near Fhb1, a major QTL for Fusarium head blight resistance on chromosome 3BS in wheat, were used to screen the Chinese Spring chromosome 3B BAC library, and a BAC contig spanning the Fhb1 region was constructed. The genic regions of two overlapping BAC clones were sequenced. Based on new DNA markers developed from the BAC sequences, Fhb1 was narrowed down to a 261 kb region with seven putative genes. The expression of the candidate genes was examined by RT-PCR. Four out of the seven genes are expressed in wheat spikes. But, there is no clear expression difference between water-inoculated and Fusarium-inoculated wheat spikes. Five cosmid clones containing all seven candidate genes were isolated from a cosmid library of Sumai 3. The cosmid clones were used to transform FHB-susceptible cultivar Bobwhite and transgenic plants were obtained for all cosmid clones. Transgenic plants for four out the five cosmid clones have been tested for Type II resistance to FHB, and none of the

Název v anglickém jazyce

Toward positional cloning of Fhb1, a major QTL for Fusarium head blight resistance in wheat

Popis výsledku anglicky

DNA markers near Fhb1, a major QTL for Fusarium head blight resistance on chromosome 3BS in wheat, were used to screen the Chinese Spring chromosome 3B BAC library, and a BAC contig spanning the Fhb1 region was constructed. The genic regions of two overlapping BAC clones were sequenced. Based on new DNA markers developed from the BAC sequences, Fhb1 was narrowed down to a 261 kb region with seven putative genes. The expression of the candidate genes was examined by RT-PCR. Four out of the seven genes are expressed in wheat spikes. But, there is no clear expression difference between water-inoculated and Fusarium-inoculated wheat spikes. Five cosmid clones containing all seven candidate genes were isolated from a cosmid library of Sumai 3. The cosmid clones were used to transform FHB-susceptible cultivar Bobwhite and transgenic plants were obtained for all cosmid clones. Transgenic plants for four out the five cosmid clones have been tested for Type II resistance to FHB, and none of the

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cereal Research Communications

ISSN

0133-3720

e-ISSN

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Svazek periodika

36

Číslo periodika v rámci svazku

suppl. B

Stát vydavatele periodika

HU - Maďarsko

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000259563800050

EID výsledku v databázi Scopus

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