Mesalamine modulates intercellular adhesion intercellular adhesion through inhibition of p-21 activated kinase-1

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F13%3A00067648" target="_blank" >RIV/00216224:14740/13:00067648 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.bcp.2012.10.026" target="_blank" >http://dx.doi.org/10.1016/j.bcp.2012.10.026</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bcp.2012.10.026" target="_blank" >10.1016/j.bcp.2012.10.026</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Mesalamine modulates intercellular adhesion intercellular adhesion through inhibition of p-21 activated kinase-1

Popis výsledku v původním jazyce

Mesalamine (5-ASA) is widely used for the treatment of ulcerative colitis, a remitting condition characterized by chronic inflammation of the colon. Knowledge about the molecular and cellular targets of 5-ASA is limited and a clear understanding of its activity in intestinal homeostasis and interference with neoplastic progression is lacking. We sought to identify molecular pathways interfered by 5-ASA, using CRC cell lines with different genetic background. Microarray was performed for gene expressionprofile of 5-ASA-treated and untreated cells (HCT116 and HT29). Filtering and analysis of data identified three oncogenic pathways interfered by 5-ASA: MAPK/ERK pathway, cell adhesion and beta-catenin/Wnt signaling. PAK1 emerged as a consensus target of5-ASA, orchestrating these pathways. We further investigated the effect of 5-ASA on cell adhesion. 5-ASA increased cell adhesion which was measured by cell adhesion assay and transcellular-resistance measurement.

Název v anglickém jazyce

Mesalamine modulates intercellular adhesion intercellular adhesion through inhibition of p-21 activated kinase-1

Popis výsledku anglicky

Mesalamine (5-ASA) is widely used for the treatment of ulcerative colitis, a remitting condition characterized by chronic inflammation of the colon. Knowledge about the molecular and cellular targets of 5-ASA is limited and a clear understanding of its activity in intestinal homeostasis and interference with neoplastic progression is lacking. We sought to identify molecular pathways interfered by 5-ASA, using CRC cell lines with different genetic background. Microarray was performed for gene expressionprofile of 5-ASA-treated and untreated cells (HCT116 and HT29). Filtering and analysis of data identified three oncogenic pathways interfered by 5-ASA: MAPK/ERK pathway, cell adhesion and beta-catenin/Wnt signaling. PAK1 emerged as a consensus target of5-ASA, orchestrating these pathways. We further investigated the effect of 5-ASA on cell adhesion. 5-ASA increased cell adhesion which was measured by cell adhesion assay and transcellular-resistance measurement.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

—

Návaznosti

O - Projekt operacniho programu

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochemical Pharmacology

ISSN

0006-2952

e-ISSN

—

Svazek periodika

85

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

234-244

Kód UT WoS článku

000314143400011

EID výsledku v databázi Scopus

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