Identification of Quantum Dots Labeled Metallothionein by Fast Scanning Laser-Induced Breakdown Spectroscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F13%3A00070556" target="_blank" >RIV/00216224:14740/13:00070556 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification of Quantum Dots Labeled Metallothionein by Fast Scanning Laser-Induced Breakdown Spectroscopy

Popis výsledku v původním jazyce

LIBS was applied for rapid determination of cadmium presented in Cd-containing QDs (CdS, CdTe), which were used for labeling of MT, anti-MT and anti-Cd antibodies. Metallothionein and both antibodies were deposited on the polystyrene surface. 2D fast scanning was used for detection of QDs labeled proteins. The detection was based on the quantification of cadmium on the emission line 508.58 nm. In order to reach high sensitivity and high spatial resolution single pulse and double pulse technique was optimized and several types of raster and line scanning modes were used. Due to the application of Nd:YAG lasers UP-266 MACRO, Brilliant and detector PI Max3 repetition rate up to 10 Hz was used for fast scanning and 2D mapping of spatial distribution cadmium on the polystyrene surface.

Název v anglickém jazyce

Identification of Quantum Dots Labeled Metallothionein by Fast Scanning Laser-Induced Breakdown Spectroscopy

Popis výsledku anglicky

LIBS was applied for rapid determination of cadmium presented in Cd-containing QDs (CdS, CdTe), which were used for labeling of MT, anti-MT and anti-Cd antibodies. Metallothionein and both antibodies were deposited on the polystyrene surface. 2D fast scanning was used for detection of QDs labeled proteins. The detection was based on the quantification of cadmium on the emission line 508.58 nm. In order to reach high sensitivity and high spatial resolution single pulse and double pulse technique was optimized and several types of raster and line scanning modes were used. Due to the application of Nd:YAG lasers UP-266 MACRO, Brilliant and detector PI Max3 repetition rate up to 10 Hz was used for fast scanning and 2D mapping of spatial distribution cadmium on the polystyrene surface.

Druh

O - Ostatní výsledky

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů