Targeted In Vivo Inhibition of Specific Protein?Protein Interactions Using Recombinant Antibodies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00074131" target="_blank" >RIV/00216224:14740/14:00074131 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0109875" target="_blank" >http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0109875</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0109875" target="_blank" >10.1371/journal.pone.0109875</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Targeted In Vivo Inhibition of Specific Protein?Protein Interactions Using Recombinant Antibodies

Popis výsledku v původním jazyce

With the growing availability of genomic sequence information, there is an increasing need for gene function analysis. Antibody-mediated ?silencing? represents an intriguing alternative for the precise inhibition of a particular function of biomolecules.Here, we describe a method for selecting recombinant antibodies with a specific purpose in mind, which is to inhibit intrinsic protein?protein interactions in the cytosol of plant cells. Experimental procedures were designed for conveniently evaluatingdesired properties of recombinant antibodies in consecutive steps. Our selection method was successfully used to develop a recombinant antibody inhibiting the interaction of ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 3 with such of its upstream interaction partners as the receiver domain of CYTOKININ INDEPENDENT HISTIDINE KINASE 1. The specific down-regulation of the cytokinin signaling pathway in vivo demonstrates the validity of our approach.

Název v anglickém jazyce

Targeted In Vivo Inhibition of Specific Protein?Protein Interactions Using Recombinant Antibodies

Popis výsledku anglicky

With the growing availability of genomic sequence information, there is an increasing need for gene function analysis. Antibody-mediated ?silencing? represents an intriguing alternative for the precise inhibition of a particular function of biomolecules.Here, we describe a method for selecting recombinant antibodies with a specific purpose in mind, which is to inhibit intrinsic protein?protein interactions in the cytosol of plant cells. Experimental procedures were designed for conveniently evaluatingdesired properties of recombinant antibodies in consecutive steps. Our selection method was successfully used to develop a recombinant antibody inhibiting the interaction of ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 3 with such of its upstream interaction partners as the receiver domain of CYTOKININ INDEPENDENT HISTIDINE KINASE 1. The specific down-regulation of the cytokinin signaling pathway in vivo demonstrates the validity of our approach.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLOS ONE : Public Library of Science

ISSN

1932-6203

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

"nestránkováno"

Kód UT WoS článku

000343941200081

EID výsledku v databázi Scopus

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