Recombinant antibodies for in vivo applications

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00073670" target="_blank" >RIV/00216224:14310/14:00073670 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.structbio.org/index.php/conferences/25-xiidiscussions" target="_blank" >http://www.structbio.org/index.php/conferences/25-xiidiscussions</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Recombinant antibodies for in vivo applications

Popis výsledku v původním jazyce

Interactions between biomolecules constitute the core of biological functions. In protein?protein interactions, specificity plays a key role and many proteins have evolved into extraordinarily precise molecular machines. The multistep phosphorylation pathway of the cytokinin signaling in plants is involving promiscuous protein-protein interactions, allowing the plasticity of the plant cell response to environmental and developmental stimuli. We have developed a recombinant antibody which is able to precisely bind to a specific protein from the phosporylation pathway in vivo. Hereinafter this protein is not phosphorylated after the signal perception and therefore the message is not delivered to the nucleus to commit the reaction of the plant. To accomplish a successful selection of the recombinant antibody, we have developed a in vivo selection method. This method allows targeting of recombinant antibodies for a precise activity. Along the way of developing our method, we came to severa

Název v anglickém jazyce

Recombinant antibodies for in vivo applications

Popis výsledku anglicky

Interactions between biomolecules constitute the core of biological functions. In protein?protein interactions, specificity plays a key role and many proteins have evolved into extraordinarily precise molecular machines. The multistep phosphorylation pathway of the cytokinin signaling in plants is involving promiscuous protein-protein interactions, allowing the plasticity of the plant cell response to environmental and developmental stimuli. We have developed a recombinant antibody which is able to precisely bind to a specific protein from the phosporylation pathway in vivo. Hereinafter this protein is not phosphorylated after the signal perception and therefore the message is not delivered to the nucleus to commit the reaction of the plant. To accomplish a successful selection of the recombinant antibody, we have developed a in vivo selection method. This method allows targeting of recombinant antibodies for a precise activity. Along the way of developing our method, we came to severa

Druh

O - Ostatní výsledky

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA521%2F09%2F1699" target="_blank" >GA521/09/1699: Imunomodulace jako nástroj funkční proteomiky při studiu cytokininové signální dráhy u Arabidopsis thaliana</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů