Stability of human telomere quadruplexes at high DNA concentrations

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00074985" target="_blank" >RIV/00216224:14740/14:00074985 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/14:00435395

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/bip.22400/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/bip.22400/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/bip.22400" target="_blank" >10.1002/bip.22400</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Stability of human telomere quadruplexes at high DNA concentrations

Popis výsledku v původním jazyce

For mimicking macromolecular crowding of DNA quadruplexes, various crowding agents have been used, typically PEG, with quadruplexes of micromolar strand concentrations. Thermal and thermodynamic stabilities of these quadruplexes increased with the concentration of the agents, the rise depended on the crowder used. A different phenomenon was observed, and is presented in this article, when the crowder was the quadruplex itself. With DNA strand concentrations ranging from 3 mM to 9 mM, the thermostabilitydid not change up to 2 mM, above which it increased, indicating that the unfolding quadruplex units were not monomolecular above 2 mM. The results are explained by self-association of the G-quadruplexes above this concentration. The deltaG(o) 37 values,evaluated only below 2 mM, did not become more negative, as with the non-DNA crowders, instead, slightly increased. Folding topology changed from antiparallel to hybrid above 2 mM, and then to parallel quadruplexes at high, 6-9 mM strand

Název v anglickém jazyce

Stability of human telomere quadruplexes at high DNA concentrations

Popis výsledku anglicky

For mimicking macromolecular crowding of DNA quadruplexes, various crowding agents have been used, typically PEG, with quadruplexes of micromolar strand concentrations. Thermal and thermodynamic stabilities of these quadruplexes increased with the concentration of the agents, the rise depended on the crowder used. A different phenomenon was observed, and is presented in this article, when the crowder was the quadruplex itself. With DNA strand concentrations ranging from 3 mM to 9 mM, the thermostabilitydid not change up to 2 mM, above which it increased, indicating that the unfolding quadruplex units were not monomolecular above 2 mM. The results are explained by self-association of the G-quadruplexes above this concentration. The deltaG(o) 37 values,evaluated only below 2 mM, did not become more negative, as with the non-DNA crowders, instead, slightly increased. Folding topology changed from antiparallel to hybrid above 2 mM, and then to parallel quadruplexes at high, 6-9 mM strand

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biopolymers

ISSN

0006-3525

e-ISSN

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Svazek periodika

101

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

428-438

Kód UT WoS článku

000336575300013

EID výsledku v databázi Scopus

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