Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00076277" target="_blank" >RIV/00216224:14740/14:00076277 - isvavai.cz</a>

Výsledek na webu

<a href="http://pubs.acs.org/doi/abs/10.1021/ac501623m" target="_blank" >http://pubs.acs.org/doi/abs/10.1021/ac501623m</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/ac501623m" target="_blank" >10.1021/ac501623m</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor

Popis výsledku v původním jazyce

Airborne microorganisms are a major cause of respiratory diseases. Detection of pathogenic bacteria in the form of bioaerosols is required not only in peacetime but also in the threat of biological attacks. The label-free and direct detection of aerosolized biological agents is presented here. A desktop bioaerosol chamber for safe work with aerosolized microbial cells was constructed, and its functionality was tested. The model organisms (Escherichia coli) were disseminated using an aerosol generator inthe chamber filled with either common laboratory indoor air or sterile air. The particles from the generated aerosol were collected using the cyclone SASS 2300, suspended in buffer and then analyzed using the piezoelectric immunosensor modified with specific capture antibodies. The frequency shifts indicated presence of the model biological agent with limit of detection of 1.45 x 104 CFU x L?1 of air. The total time from sample collection to detection was 16 min.

Název v anglickém jazyce

Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor

Popis výsledku anglicky

Airborne microorganisms are a major cause of respiratory diseases. Detection of pathogenic bacteria in the form of bioaerosols is required not only in peacetime but also in the threat of biological attacks. The label-free and direct detection of aerosolized biological agents is presented here. A desktop bioaerosol chamber for safe work with aerosolized microbial cells was constructed, and its functionality was tested. The model organisms (Escherichia coli) were disseminated using an aerosol generator inthe chamber filled with either common laboratory indoor air or sterile air. The particles from the generated aerosol were collected using the cyclone SASS 2300, suspended in buffer and then analyzed using the piezoelectric immunosensor modified with specific capture antibodies. The frequency shifts indicated presence of the model biological agent with limit of detection of 1.45 x 104 CFU x L?1 of air. The total time from sample collection to detection was 16 min.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Chemistry

ISSN

0003-2700

e-ISSN

—

Svazek periodika

86

Číslo periodika v rámci svazku

17

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

8680-8686

Kód UT WoS článku

000341229200026

EID výsledku v databázi Scopus

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