Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00082954" target="_blank" >RIV/00216224:14740/15:00082954 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1117/12.2084606" target="_blank" >http://dx.doi.org/10.1117/12.2084606</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1117/12.2084606" target="_blank" >10.1117/12.2084606</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms
Popis výsledku v původním jazyce
Biological warfare agents (BWAs) represent the current menace of the asymmetric war. The early detection of BWAs, especially in the form of bioaerosol, is a challenging task for governments all around the world. Label-free quartz crystal microbalance (QCM) immunosensor and electrochemical immunosensor were developed and tested for rapid detection of BWA surrogate (E. coli) in the form of bioaerosol. Two immobilization strategies for the attachment of antibody were tested; the gold sensor surface was activated by cysteamine and then antibody was covalently linked either using glutaraldehyde, or the reduced antibodies were attached via Sulfo-SMCC. A portable bioaerosol chamber was constructed and used for safe manipulation with aerosolized microorganisms. The dissemination was done using a piezoelectric humidifier, distribution of bioaerosol inside the chamber was ensured using three 12-cm fans. The whole system was controlled remotely using LAN network.
Název v anglickém jazyce
Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms
Popis výsledku anglicky
Biological warfare agents (BWAs) represent the current menace of the asymmetric war. The early detection of BWAs, especially in the form of bioaerosol, is a challenging task for governments all around the world. Label-free quartz crystal microbalance (QCM) immunosensor and electrochemical immunosensor were developed and tested for rapid detection of BWA surrogate (E. coli) in the form of bioaerosol. Two immobilization strategies for the attachment of antibody were tested; the gold sensor surface was activated by cysteamine and then antibody was covalently linked either using glutaraldehyde, or the reduced antibodies were attached via Sulfo-SMCC. A portable bioaerosol chamber was constructed and used for safe manipulation with aerosolized microorganisms. The dissemination was done using a piezoelectric humidifier, distribution of bioaerosol inside the chamber was ensured using three 12-cm fans. The whole system was controlled remotely using LAN network.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
CE - Biochemie
OECD FORD obor
—
Návaznosti výsledku
Projekt
<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI
ISBN
9781628415711
ISSN
0277-786X
e-ISSN
—
Počet stran výsledku
9
Strana od-do
1-9
Název nakladatele
SPIE
Místo vydání
USA
Místo konání akce
Baltimore, MD, USA
Datum konání akce
20. 4. 2015
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
000357744700006