Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5? in Bioaerosols

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00000493%3AG41__%2F11%3A%230000388" target="_blank" >RIV/00000493:G41__/11:#0000388 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5? in Bioaerosols

Popis výsledku v původním jazyce

The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5? in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 103 to 108?CFU/mL within 30?min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector.The level of 150?CFU/L in air was clearly indicated (20?min analysis including 5?min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions.

Název v anglickém jazyce

Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5? in Bioaerosols

Popis výsledku anglicky

The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5? in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 103 to 108?CFU/mL within 30?min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector.The level of 150?CFU/L in air was clearly indicated (20?min analysis including 5?min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/OVVTUO2008001" target="_blank" >OVVTUO2008001: NANOBIO - Nanotechnologie pro imunosenzorovou detekci bioaerosolů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electroanalysis

ISSN

1040-0397

e-ISSN

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Svazek periodika

24

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

539-546

Kód UT WoS článku

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EID výsledku v databázi Scopus

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