Gas-assisted annular microsprayer for sample preparation for time-resolved cryo-electron microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00079215" target="_blank" >RIV/00216224:14740/14:00079215 - isvavai.cz</a>

Výsledek na webu

<a href="http://iopscience.iop.org/0960-1317/24/11/115001/pdf/0960-1317_24_11_115001.pdf" target="_blank" >http://iopscience.iop.org/0960-1317/24/11/115001/pdf/0960-1317_24_11_115001.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/0960-1317/24/11/115001" target="_blank" >10.1088/0960-1317/24/11/115001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Gas-assisted annular microsprayer for sample preparation for time-resolved cryo-electron microscopy

Popis výsledku v původním jazyce

Time-resolved cryo-electron microscopy (TRCEM) has emerged as a powerful technique for transient structural characterization of isolated biomacromolecular complexes in their native state within the time scale of seconds to milliseconds. For TRCEM samplepreparation, a microfluidic device has been demonstrated to be a promising approach to facilitate TRCEM biological sample preparation. It is capable of achieving rapidly aqueous sample mixing, controlled reaction incubation, and sample deposition on electron microscopy (EM) grids for rapid freezing. One of the critical challenges is to transfer samples to cryo-EM grids from the microfluidic device. By using a microspraying method, the generated droplet size needs to be controlled to facilitate thin icefilm formation on the grid surface for efficient data collection, whilst not being so thin that it dries out before freezing, i.e. an optimized mean droplet size needs to be achieved.

Název v anglickém jazyce

Gas-assisted annular microsprayer for sample preparation for time-resolved cryo-electron microscopy

Popis výsledku anglicky

Time-resolved cryo-electron microscopy (TRCEM) has emerged as a powerful technique for transient structural characterization of isolated biomacromolecular complexes in their native state within the time scale of seconds to milliseconds. For TRCEM samplepreparation, a microfluidic device has been demonstrated to be a promising approach to facilitate TRCEM biological sample preparation. It is capable of achieving rapidly aqueous sample mixing, controlled reaction incubation, and sample deposition on electron microscopy (EM) grids for rapid freezing. One of the critical challenges is to transfer samples to cryo-EM grids from the microfluidic device. By using a microspraying method, the generated droplet size needs to be controlled to facilitate thin icefilm formation on the grid surface for efficient data collection, whilst not being so thin that it dries out before freezing, i.e. an optimized mean droplet size needs to be achieved.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF MICROMECHANICS AND MICROENGINEERING

ISSN

0960-1317

e-ISSN

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Svazek periodika

24

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

"nestránkováno"

Kód UT WoS článku

000345262800001

EID výsledku v databázi Scopus

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