Deveopment of a microfluidic specimenpreparation device for time-resolved cryo-electron microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00094149" target="_blank" >RIV/00216224:14740/16:00094149 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Deveopment of a microfluidic specimenpreparation device for time-resolved cryo-electron microscopy

Popis výsledku v původním jazyce

The goal of structural biology is to be able to understand how macromolecular complex functions. In traditional, non-time-resolved cryoelectron microscopy (cryo-EM), this is accomplished by determination of static structures. Existing time-resolved techniques are difficult or inefficient, and thus have been rarely used. The goal of this proposal is to construct a customized cryo-preparation device specifically for the microfluidics-based time-resolved method that I previously helped develop. The steps required for time-resolved cryo-EM are, as quickly as possible: 1) to initiate a chemical reaction, 2) to form an aqueous layer thin enough to transmit the electron beam, and 3) to arrest the chemical reaction in cryogen. The microfluidics-based method makes use of a device which mixes two components, sprays the mixture onto an EM grid, and then plunges the grid into cryogen.

Název v anglickém jazyce

Deveopment of a microfluidic specimenpreparation device for time-resolved cryo-electron microscopy

Popis výsledku anglicky

The goal of structural biology is to be able to understand how macromolecular complex functions. In traditional, non-time-resolved cryoelectron microscopy (cryo-EM), this is accomplished by determination of static structures. Existing time-resolved techniques are difficult or inefficient, and thus have been rarely used. The goal of this proposal is to construct a customized cryo-preparation device specifically for the microfluidics-based time-resolved method that I previously helped develop. The steps required for time-resolved cryo-EM are, as quickly as possible: 1) to initiate a chemical reaction, 2) to form an aqueous layer thin enough to transmit the electron beam, and 3) to arrest the chemical reaction in cryogen. The microfluidics-based method makes use of a device which mixes two components, sprays the mixture onto an EM grid, and then plunges the grid into cryogen.

Druh

V_souhrn - Souhrnná výzkumná zpráva

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2016

Kód důvěrnosti údajů

C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.

Počet stran výsledku

3

Místo vydání

Brno

Název nakladatele resp. objednatele

Instruct Academic Services Ltd.

Verze

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