Age-Related Decrease in TCR Repertoire Diversity Measured with Deep and Normalized Sequence Profiling
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00079244" target="_blank" >RIV/00216224:14740/14:00079244 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.4049/jimmunol.1302064" target="_blank" >http://dx.doi.org/10.4049/jimmunol.1302064</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4049/jimmunol.1302064" target="_blank" >10.4049/jimmunol.1302064</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Age-Related Decrease in TCR Repertoire Diversity Measured with Deep and Normalized Sequence Profiling
Popis výsledku v původním jazyce
The decrease of TCR diversity with aging has never been studied by direct methods. In this study, we combined high-throughput Illumina sequencing with unique cDNA molecular identifier technology to achieve deep and precisely normalized profiling of TCR beta repertoires in 39 healthy donors aged 6-90 y. We demonstrate that TCR beta diversity per 10(6) T cells decreases roughly linearly with age, with significant reduction already apparent by age 40. The percentage of naive T cells showed a strong correlation with measured TCR diversity and decreased linearly up to age 70. Remarkably, the oldest group (average age 82 y) was characterized by a higher percentage of naive CD4(+) T cells, lower abundance of expanded clones, and increased TCR diversity compared with the previous age group (average age 62 y), suggesting the influence of age selection and association of these three related parameters with longevity.
Název v anglickém jazyce
Age-Related Decrease in TCR Repertoire Diversity Measured with Deep and Normalized Sequence Profiling
Popis výsledku anglicky
The decrease of TCR diversity with aging has never been studied by direct methods. In this study, we combined high-throughput Illumina sequencing with unique cDNA molecular identifier technology to achieve deep and precisely normalized profiling of TCR beta repertoires in 39 healthy donors aged 6-90 y. We demonstrate that TCR beta diversity per 10(6) T cells decreases roughly linearly with age, with significant reduction already apparent by age 40. The percentage of naive T cells showed a strong correlation with measured TCR diversity and decreased linearly up to age 70. Remarkably, the oldest group (average age 82 y) was characterized by a higher percentage of naive CD4(+) T cells, lower abundance of expanded clones, and increased TCR diversity compared with the previous age group (average age 62 y), suggesting the influence of age selection and association of these three related parameters with longevity.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FB - Endokrinologie, diabetologie, metabolismus, výživa
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of immunology
ISSN
0022-1767
e-ISSN
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Svazek periodika
192
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
10
Strana od-do
2689-2698
Kód UT WoS článku
000332702700018
EID výsledku v databázi Scopus
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