Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00081165" target="_blank" >RIV/00216224:14740/15:00081165 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/15:00447356

Výsledek na webu

<a href="http://jxb.oxfordjournals.org/content/66/16/5055.abstract" target="_blank" >http://jxb.oxfordjournals.org/content/66/16/5055.abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/erv177" target="_blank" >10.1093/jxb/erv177</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles

Popis výsledku v původním jazyce

The plant hormone auxin is a key regulator of plant growth and development. Auxin levels are sensed and interpreted by distinct receptor systems that activate a broad range of cellular responses. The Auxin-Binding Protein1 (ABP1) that has been identifiedbased on its ability to bind auxin with high affinity is a prime candidate for the extracellular receptor responsible for mediating a range of auxin effects, in particular, the fast non-transcriptional ones. Contradictory genetic studies suggested prominent or no importance of ABP1 in many developmental processes. However, how crucial the role of auxin binding to ABP1 is for its functions has not been addressed. Here, we show that the auxinbinding pocket of ABP1 is essential for its gain-of-function cellular and developmental roles. In total, 16 different abp1 mutants were prepared that possessed substitutions in the metal core or in the hydrophobic amino acids of the auxin-binding pocket as well as neutral mutations.

Název v anglickém jazyce

Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles

Popis výsledku anglicky

The plant hormone auxin is a key regulator of plant growth and development. Auxin levels are sensed and interpreted by distinct receptor systems that activate a broad range of cellular responses. The Auxin-Binding Protein1 (ABP1) that has been identifiedbased on its ability to bind auxin with high affinity is a prime candidate for the extracellular receptor responsible for mediating a range of auxin effects, in particular, the fast non-transcriptional ones. Contradictory genetic studies suggested prominent or no importance of ABP1 in many developmental processes. However, how crucial the role of auxin binding to ABP1 is for its functions has not been addressed. Here, we show that the auxinbinding pocket of ABP1 is essential for its gain-of-function cellular and developmental roles. In total, 16 different abp1 mutants were prepared that possessed substitutions in the metal core or in the hydrophobic amino acids of the auxin-binding pocket as well as neutral mutations.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

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Svazek periodika

66

Číslo periodika v rámci svazku

16

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

5055-5065

Kód UT WoS článku

000359688300017

EID výsledku v databázi Scopus

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