Developmental roles of Auxin Binding Protein 1 in Arabidopsis thaliana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00541512" target="_blank" >RIV/61389030:_____/21:00541512 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/21:00118780 RIV/00216208:11310/21:10439320 RIV/61989592:15310/21:73610741

Výsledek na webu

<a href="http://doi.org/10.1016/j.plantsci.2020.110750" target="_blank" >http://doi.org/10.1016/j.plantsci.2020.110750</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plantsci.2020.110750" target="_blank" >10.1016/j.plantsci.2020.110750</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Developmental roles of Auxin Binding Protein 1 in Arabidopsis thaliana

Popis výsledku v původním jazyce

Auxin is a major plant growth regulator, but current models on auxin perception and signaling cannot explain the whole plethora of auxin effects, in particular those associated with rapid responses. A possible candidate for a component of additional auxin perception mechanisms is the AUXIN BINDING PROTEIN 1 (ABP1), whose function in planta remains unclear. Here we combined expression analysis with gain- and loss-of-function approaches to analyze the role of ABP1 in plant development. ABP1 shows a broad expression largely overlapping with, but not regulated by, transcriptional auxin response activity. Furthermore, ABP1 activity is not essential for the transcriptional auxin signaling. Genetic in planta analysis revealed that abp1 loss-of-function mutants show largely normal development with minor defects in bolting. On the other hand, ABP1 gain-of-function alleles show a broad range of growth and developmental defects, including root and hypocotyl growth and bending, lateral root and leaf development, bolting, as well as response to heat stress. At the cellular level, ABP1 gain-of-function leads to impaired auxin effect on PIN polar distribution and affects BFA-sensitive PIN intracellular aggregation. The gain-of-function analysis suggests a broad, but still mechanistically unclear involvement of ABP1 in plant development, possibly masked in abp1 loss-of-function mutants by a functional redundancy.

Název v anglickém jazyce

Developmental roles of Auxin Binding Protein 1 in Arabidopsis thaliana

Popis výsledku anglicky

Auxin is a major plant growth regulator, but current models on auxin perception and signaling cannot explain the whole plethora of auxin effects, in particular those associated with rapid responses. A possible candidate for a component of additional auxin perception mechanisms is the AUXIN BINDING PROTEIN 1 (ABP1), whose function in planta remains unclear. Here we combined expression analysis with gain- and loss-of-function approaches to analyze the role of ABP1 in plant development. ABP1 shows a broad expression largely overlapping with, but not regulated by, transcriptional auxin response activity. Furthermore, ABP1 activity is not essential for the transcriptional auxin signaling. Genetic in planta analysis revealed that abp1 loss-of-function mutants show largely normal development with minor defects in bolting. On the other hand, ABP1 gain-of-function alleles show a broad range of growth and developmental defects, including root and hypocotyl growth and bending, lateral root and leaf development, bolting, as well as response to heat stress. At the cellular level, ABP1 gain-of-function leads to impaired auxin effect on PIN polar distribution and affects BFA-sensitive PIN intracellular aggregation. The gain-of-function analysis suggests a broad, but still mechanistically unclear involvement of ABP1 in plant development, possibly masked in abp1 loss-of-function mutants by a functional redundancy.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Science

ISSN

0168-9452

e-ISSN

1873-2259

Svazek periodika

303

Číslo periodika v rámci svazku

FEB

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

17

Strana od-do

110750

Kód UT WoS článku

000614154500001

EID výsledku v databázi Scopus

2-s2.0-85097214684