Single-breath analysis using a novel simple sampler and capillary electrophoresis with contactless conductometric detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00081636" target="_blank" >RIV/00216224:14740/15:00081636 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/elps.201400456/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/elps.201400456/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201400456" target="_blank" >10.1002/elps.201400456</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Single-breath analysis using a novel simple sampler and capillary electrophoresis with contactless conductometric detection

Popis výsledku v původním jazyce

The analysis of ionic content of exhaled breath condensate (EBC) from one single breath by CE with (CD)-D-4 is demonstrated for the first time. A miniature sampler made from a 2-mL syringe and an aluminum cooling cylinder for collection of EBC was developed. Various parameters of the sampler that influence its collection efficiency, repeatability, and effect of respiratory patterns were studied in detail. Efficient procedures for the cleanup of the miniature sampler were also developed and resulted in significant improvement of sampling repeatability. Analysis of EBC was performed by CE-(CD)-D-4 in a 60 mM MES/L-histidine BGE with 30 mu M CTAB and 2 mM 18-crown-6 at pH 6 and excellent repeatability of migration times (RSD &lt; 1.3% (n = 7)) and peak areas (RSD &lt; 7% (n = 7)) of 12 inorganic anions, cations, and organic acids was obtained. It has been shown that the breathing pattern has a significant impact on the concentration of the analytes in the collected EBC.

Název v anglickém jazyce

Single-breath analysis using a novel simple sampler and capillary electrophoresis with contactless conductometric detection

Popis výsledku anglicky

The analysis of ionic content of exhaled breath condensate (EBC) from one single breath by CE with (CD)-D-4 is demonstrated for the first time. A miniature sampler made from a 2-mL syringe and an aluminum cooling cylinder for collection of EBC was developed. Various parameters of the sampler that influence its collection efficiency, repeatability, and effect of respiratory patterns were studied in detail. Efficient procedures for the cleanup of the miniature sampler were also developed and resulted in significant improvement of sampling repeatability. Analysis of EBC was performed by CE-(CD)-D-4 in a 60 mM MES/L-histidine BGE with 30 mu M CTAB and 2 mM 18-crown-6 at pH 6 and excellent repeatability of migration times (RSD &lt; 1.3% (n = 7)) and peak areas (RSD &lt; 7% (n = 7)) of 12 inorganic anions, cations, and organic acids was obtained. It has been shown that the breathing pattern has a significant impact on the concentration of the analytes in the collected EBC.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

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Svazek periodika

36

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

526-533

Kód UT WoS článku

000349969700006

EID výsledku v databázi Scopus

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