CAPILLARY ELECTROPHORETIC ANALYSIS OF EXHALED BREATH CONDENSATE IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00106949" target="_blank" >RIV/00216224:14740/18:00106949 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/65269705:_____/18:00069837

Výsledek na webu

<a href="http://dx.doi.org/10.1515/hjic-2018-0006" target="_blank" >http://dx.doi.org/10.1515/hjic-2018-0006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/hjic-2018-0006" target="_blank" >10.1515/hjic-2018-0006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

CAPILLARY ELECTROPHORETIC ANALYSIS OF EXHALED BREATH CONDENSATE IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE

Popis výsledku v původním jazyce

In this work, capillary electrophoresis with contactless conductometric detection (CCD) was used for the analysis of the ionic content of exhaled breath condensate (EBC) to differentiate between healthy individuals and patients with gastroesophageal reflux disease (GERD). The exhaled breath condensate was collected using a miniature sample collection device and the content analyzed using a separation electrolyte composed of 20 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM L-histidine, 2 mM 18-Crown-6 and 30 mu M cetyltrimethylammonium bromide. The separation of anions took less than 2.5 minutes, while the cations were separated in less than 1.5 minutes. The most significantly elevated ions in the group of patients suffering from gastroesophageal reflux disease were chloride, nitrate, propionate and butyrate. Although the number of subjects was too small to draw definite conclusions with regard to the discriminatory power of these ions, the pilot data are promising for EBC as a useful non-invasive alternative for other methods used in the diagnosis of gastroesophageal reflux disease.

Název v anglickém jazyce

CAPILLARY ELECTROPHORETIC ANALYSIS OF EXHALED BREATH CONDENSATE IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE

Popis výsledku anglicky

In this work, capillary electrophoresis with contactless conductometric detection (CCD) was used for the analysis of the ionic content of exhaled breath condensate (EBC) to differentiate between healthy individuals and patients with gastroesophageal reflux disease (GERD). The exhaled breath condensate was collected using a miniature sample collection device and the content analyzed using a separation electrolyte composed of 20 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM L-histidine, 2 mM 18-Crown-6 and 30 mu M cetyltrimethylammonium bromide. The separation of anions took less than 2.5 minutes, while the cations were separated in less than 1.5 minutes. The most significantly elevated ions in the group of patients suffering from gastroesophageal reflux disease were chloride, nitrate, propionate and butyrate. Although the number of subjects was too small to draw definite conclusions with regard to the discriminatory power of these ions, the pilot data are promising for EBC as a useful non-invasive alternative for other methods used in the diagnosis of gastroesophageal reflux disease.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/NV17-31945A" target="_blank" >NV17-31945A: Vývoj neinvazivní diagnostiky extraezofageálních projevů refluxní choroby jícnu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY

ISSN

0133-0276

e-ISSN

—

Svazek periodika

46

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

5

Strana od-do

23-27

Kód UT WoS článku

000440079700006

EID výsledku v databázi Scopus

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