Carboxyl-rich plasma polymer surfaces in surface plasmon resonance immunosensing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00102139" target="_blank" >RIV/00216224:14740/18:00102139 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.7567/JJAP.57.01AG06" target="_blank" >http://dx.doi.org/10.7567/JJAP.57.01AG06</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.7567/JJAP.57.01AG06" target="_blank" >10.7567/JJAP.57.01AG06</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Carboxyl-rich plasma polymer surfaces in surface plasmon resonance immunosensing

Popis výsledku v původním jazyce

Stable carboxyl-rich plasma polymers (PPs) were deposited onto the gold surface of surface plasmon resonance (SPR) chips under conditions that were chosen based on lumped kinetic model results. Carboxyl-rich films are of high interest for bio-applications thanks to their high reactivity, allowing the formation of covalent linkages between biomolecules and a surface. Accordingly, the monoclonal antibody, specific to human serum albumin (HSA), was immobilized and the performance of SPR immunosensors was evaluated by the immunoassay flow test. The developed sensors performed high level of stability and provided selective and high response to the HSA antigen solutions. The achieved results confirmed that the presented methodologies for the grafting of biomolecules on the gold surfaces have great potential for biosensing applications.

Název v anglickém jazyce

Carboxyl-rich plasma polymer surfaces in surface plasmon resonance immunosensing

Popis výsledku anglicky

Stable carboxyl-rich plasma polymers (PPs) were deposited onto the gold surface of surface plasmon resonance (SPR) chips under conditions that were chosen based on lumped kinetic model results. Carboxyl-rich films are of high interest for bio-applications thanks to their high reactivity, allowing the formation of covalent linkages between biomolecules and a surface. Accordingly, the monoclonal antibody, specific to human serum albumin (HSA), was immobilized and the performance of SPR immunosensors was evaluated by the immunoassay flow test. The developed sensors performed high level of stability and provided selective and high response to the HSA antigen solutions. The achieved results confirmed that the presented methodologies for the grafting of biomolecules on the gold surfaces have great potential for biosensing applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10305 - Fluids and plasma physics (including surface physics)

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Japanese Journal of Applied Physics

ISSN

0021-4922

e-ISSN

—

Svazek periodika

57

Číslo periodika v rámci svazku

01AG06

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

„01AG06-1“-„01AG06-5“

Kód UT WoS článku

000418412100048

EID výsledku v databázi Scopus

2-s2.0-85039999151