A Surprising Photoactivity of Blue Fluorescent Protein TagBFP Allows for Super-Resolution Microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00106647" target="_blank" >RIV/00216224:14740/18:00106647 - isvavai.cz</a>

Výsledek na webu

<a href="https://journals.aps.org/prl/abstract/10.1103/PhysRevLett.120.233901" target="_blank" >https://journals.aps.org/prl/abstract/10.1103/PhysRevLett.120.233901</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17691/stm2018.10.1.04" target="_blank" >10.17691/stm2018.10.1.04</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A Surprising Photoactivity of Blue Fluorescent Protein TagBFP Allows for Super-Resolution Microscopy

Popis výsledku v původním jazyce

The aim of this research was to study photobehavior of a popular blue fluorescent protein TagBFP and apply this marker for super-resolution microscopy. Materials and Methods. Photoactivation of TagBFP was examined both in protein solution in vitro and in living cells. Subdiffraction imaging was performed using total internal reflection fluorescence microscopy followed by super-resolution radial fluctuations or single-molecule localization analysis. Results. We show that TagBFP exhibits blinking behavior upon 405 nm light illumination. Moreover, photoactivation to red-emitting state is occurring in the conditions typically used for TagBFP imaging. The red (photoactivated) form of TagBFP possesses spectral properties similar to TagRFP - a close homologue of TagBFP. We show that both blinking and photoactivation of TagBFP can be utilized for super-resolution imaging. We conclude that photoactivation of TagBFP to red-emitting form should be taken into account in the design of multi-channel imaging experiments involving high-power or prolonged UV illumination.

Název v anglickém jazyce

A Surprising Photoactivity of Blue Fluorescent Protein TagBFP Allows for Super-Resolution Microscopy

Popis výsledku anglicky

The aim of this research was to study photobehavior of a popular blue fluorescent protein TagBFP and apply this marker for super-resolution microscopy. Materials and Methods. Photoactivation of TagBFP was examined both in protein solution in vitro and in living cells. Subdiffraction imaging was performed using total internal reflection fluorescence microscopy followed by super-resolution radial fluctuations or single-molecule localization analysis. Results. We show that TagBFP exhibits blinking behavior upon 405 nm light illumination. Moreover, photoactivation to red-emitting state is occurring in the conditions typically used for TagBFP imaging. The red (photoactivated) form of TagBFP possesses spectral properties similar to TagRFP - a close homologue of TagBFP. We show that both blinking and photoactivation of TagBFP can be utilized for super-resolution imaging. We conclude that photoactivation of TagBFP to red-emitting form should be taken into account in the design of multi-channel imaging experiments involving high-power or prolonged UV illumination.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30102 - Immunology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

SOVREMENNYE TEHNOLOGII V MEDICINE

ISSN

2076-4243

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

RU - Ruská federace

Počet stran výsledku

4

Strana od-do

35-38

Kód UT WoS článku

000431017800004

EID výsledku v databázi Scopus

2-s2.0-85044465258