CXCR3 Identifies Human Naive CD8(+) T Cells with Enhanced Effector Differentiation Potential

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F19%3A00113376" target="_blank" >RIV/00216224:14740/19:00113376 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.jimmunol.org/content/203/12/3179" target="_blank" >https://www.jimmunol.org/content/203/12/3179</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4049/jimmunol.1901072" target="_blank" >10.4049/jimmunol.1901072</a>

Jazyk výsledku

angličtina

Název v původním jazyce

CXCR3 Identifies Human Naive CD8(+) T Cells with Enhanced Effector Differentiation Potential

Popis výsledku v původním jazyce

In mice, the ability of naive T (T-N) cells to mount an effector response correlates with TCR sensitivity for self-derived Ags, which can be quantified indirectly by measuring surface expression levels of CD5. Equivalent findings have not been reported previously in humans. We identified two discrete subsets of human CD8(+) T-N cells, defined by the absence or presence of the chemokine receptor CXCR3. The more abundant CXCR3(+) T-N cell subset displayed an effector-like transcriptional profile and expressed TCRs with physicochemical characteristics indicative of enhanced interactions with peptide-HLA class I Ags. Moreover, CXCR3(+) T-N cells frequently produced IL-2 and TNF in response to nonspecific activation directly ex vivo and differentiated readily into Ag-specific effector cells in vitro. Comparative analyses further revealed that human CXCR3(+) T-N cells were transcriptionally equivalent to murine CXCR3(+) T-N cells, which expressed high levels of CD5. These findings provide support for the notion that effector differentiation is shaped by heterogeneity in the preimmune repertoire of human CD8(+) T cells.

Název v anglickém jazyce

CXCR3 Identifies Human Naive CD8(+) T Cells with Enhanced Effector Differentiation Potential

Popis výsledku anglicky

In mice, the ability of naive T (T-N) cells to mount an effector response correlates with TCR sensitivity for self-derived Ags, which can be quantified indirectly by measuring surface expression levels of CD5. Equivalent findings have not been reported previously in humans. We identified two discrete subsets of human CD8(+) T-N cells, defined by the absence or presence of the chemokine receptor CXCR3. The more abundant CXCR3(+) T-N cell subset displayed an effector-like transcriptional profile and expressed TCRs with physicochemical characteristics indicative of enhanced interactions with peptide-HLA class I Ags. Moreover, CXCR3(+) T-N cells frequently produced IL-2 and TNF in response to nonspecific activation directly ex vivo and differentiated readily into Ag-specific effector cells in vitro. Comparative analyses further revealed that human CXCR3(+) T-N cells were transcriptionally equivalent to murine CXCR3(+) T-N cells, which expressed high levels of CD5. These findings provide support for the notion that effector differentiation is shaped by heterogeneity in the preimmune repertoire of human CD8(+) T cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30102 - Immunology

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of immunology

ISSN

0022-1767

e-ISSN

—

Svazek periodika

203

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

3179-3189

Kód UT WoS článku

000501838100012

EID výsledku v databázi Scopus

2-s2.0-85076332121