BIO-APPLICABLE METAL NANOPARTICLES

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00120018" target="_blank" >RIV/00216224:14740/21:00120018 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.confer.cz/nanocon/2020" target="_blank" >https://www.confer.cz/nanocon/2020</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2020.3736" target="_blank" >10.37904/nanocon.2020.3736</a>

Jazyk výsledku

angličtina

Název v původním jazyce

BIO-APPLICABLE METAL NANOPARTICLES

Popis výsledku v původním jazyce

Metal nanoparticles find wide application potential in the biological research due to their unique optical, magnetic, electrical properties. Utilization of the nanoparticles prepared by chemical synthesis for biological research is limited by its solubility in water and toxicity. One potential solution to overcome this limitation is encapsulation of metal nanoparticles into protein or lipidic shell. Apoferritin is highly symmetric 12 nm protein cage composed of 24 apoferritin monomers which forms hollow structure with 8 nm cavity. Protein shell specifically interacts with the receptor on the cell membrane which facilitates uptake of the apoferritin from the extra-cellular environment into the cell. Due to the high stability of the protein cage and inherent capacity to encompass metal nanoparticles, apoferritin is widely focused as a potentionally optimal and general system for delivery of the metal nanoparticles into the organism. In addition, apoferritin can be also used as a nanoreactor for the nanoparticle production. We have generated a set of different nanoparticles encapsulated in apoferritin cage which we have intended to use as a label for structural and cellular biology research by cryo-electron microscopy. Here, we show that despite the 8 nm cavity, single 6-8 nm nanoparticle is not formed inside apoferritin cage. Instead, larger number of 2 nm or smaller nanoparticles is present inside apoferritin reducing overall number of atoms which can be incorporated into the molecule and thus the attainable contrast during electron microscopy imaging. Despite its wide utilization in life-science research, we conclude, that apoferritin derived nanoparticle system is not an optimal labeling probe for cryo-electron microscopy.

Název v anglickém jazyce

BIO-APPLICABLE METAL NANOPARTICLES

Popis výsledku anglicky

Metal nanoparticles find wide application potential in the biological research due to their unique optical, magnetic, electrical properties. Utilization of the nanoparticles prepared by chemical synthesis for biological research is limited by its solubility in water and toxicity. One potential solution to overcome this limitation is encapsulation of metal nanoparticles into protein or lipidic shell. Apoferritin is highly symmetric 12 nm protein cage composed of 24 apoferritin monomers which forms hollow structure with 8 nm cavity. Protein shell specifically interacts with the receptor on the cell membrane which facilitates uptake of the apoferritin from the extra-cellular environment into the cell. Due to the high stability of the protein cage and inherent capacity to encompass metal nanoparticles, apoferritin is widely focused as a potentionally optimal and general system for delivery of the metal nanoparticles into the organism. In addition, apoferritin can be also used as a nanoreactor for the nanoparticle production. We have generated a set of different nanoparticles encapsulated in apoferritin cage which we have intended to use as a label for structural and cellular biology research by cryo-electron microscopy. Here, we show that despite the 8 nm cavity, single 6-8 nm nanoparticle is not formed inside apoferritin cage. Instead, larger number of 2 nm or smaller nanoparticles is present inside apoferritin reducing overall number of atoms which can be incorporated into the molecule and thus the attainable contrast during electron microscopy imaging. Despite its wide utilization in life-science research, we conclude, that apoferritin derived nanoparticle system is not an optimal labeling probe for cryo-electron microscopy.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10300 - Physical sciences

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

CONFERENCE PROCEEDINGS - NANOCON 2020

ISBN

9788087294987

ISSN

2694-930X

e-ISSN

—

Počet stran výsledku

8

Strana od-do

347-354

Název nakladatele

TANGER LTD

Místo vydání

SLEZSKA

Místo konání akce

Brno

Datum konání akce

1. 1. 2020

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000664505500059