14-3-3ζ phosphomimicking mutants: an alternative to phosphorylation?
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00134994" target="_blank" >RIV/00216224:14740/21:00134994 - isvavai.cz</a>
Výsledek na webu
<a href="https://fns.uniba.sk/fileadmin/prif/svk/zborniky/Zbornik2021.pdf" target="_blank" >https://fns.uniba.sk/fileadmin/prif/svk/zborniky/Zbornik2021.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
14-3-3ζ phosphomimicking mutants: an alternative to phosphorylation?
Popis výsledku v původním jazyce
The 14-3-3 proteins are typically found in dimeric form crucial for their proper function in cells. Yet, a unique role of monomers has been debated in recent studies. Monomerization of 14-3-3ζ is regulated via phosphorylation of Ser58 located at dimer interface. However, it is difficult to obtain 14-3-3ζ specifically phosphorylated at Ser58 (pS58) in sufficient purity and amount. For these reasons three distinct phosphomimicking mutants, i. e. 14-3-3ζ S58E, S58D and S57D S58D were designed and studied in terms of the oligomeric nature and thermodynamical properties. Finally, they were compared to 14-3-3ζ wild type and monomeric mutant L12E M78K, used to approximate monomeric 14-3-3ζ pS58, to discuss whether they could be valid alternative for study of 14-3-3ζ pS58. Although phosphomimicking mutants S58E and S58D and 14-3-3ζ pS58 share negative charge at position 58, they were observed to exhibit distinct properties than monomeric 14-3-3ζ. Designed double mutant S57D S58D with additional negative charge in the Ser58 surrounding appeared as more appropriate alternative of pS58 protein.
Název v anglickém jazyce
14-3-3ζ phosphomimicking mutants: an alternative to phosphorylation?
Popis výsledku anglicky
The 14-3-3 proteins are typically found in dimeric form crucial for their proper function in cells. Yet, a unique role of monomers has been debated in recent studies. Monomerization of 14-3-3ζ is regulated via phosphorylation of Ser58 located at dimer interface. However, it is difficult to obtain 14-3-3ζ specifically phosphorylated at Ser58 (pS58) in sufficient purity and amount. For these reasons three distinct phosphomimicking mutants, i. e. 14-3-3ζ S58E, S58D and S57D S58D were designed and studied in terms of the oligomeric nature and thermodynamical properties. Finally, they were compared to 14-3-3ζ wild type and monomeric mutant L12E M78K, used to approximate monomeric 14-3-3ζ pS58, to discuss whether they could be valid alternative for study of 14-3-3ζ pS58. Although phosphomimicking mutants S58E and S58D and 14-3-3ζ pS58 share negative charge at position 58, they were observed to exhibit distinct properties than monomeric 14-3-3ζ. Designed double mutant S57D S58D with additional negative charge in the Ser58 surrounding appeared as more appropriate alternative of pS58 protein.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
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OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
Študentská vedecká konferencia PriF UK 2021: zborník recenzovaných príspevkov
ISBN
9788022351324
ISSN
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e-ISSN
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Počet stran výsledku
6
Strana od-do
703-708
Název nakladatele
Univerzita Komenského v Bratislave
Místo vydání
Bratislava
Místo konání akce
Bratislava
Datum konání akce
21. 4. 2021
Typ akce podle státní příslušnosti
EUR - Evropská akce
Kód UT WoS článku
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