Cycles of satellite and transposon evolution in <i>Arabidopsis</i> centromeres

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F23%3A00134414" target="_blank" >RIV/00216224:14740/23:00134414 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.nature.com/articles/s41586-023-06062-z#citeas" target="_blank" >https://www.nature.com/articles/s41586-023-06062-z#citeas</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41586-023-06062-z" target="_blank" >10.1038/s41586-023-06062-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cycles of satellite and transposon evolution in <i>Arabidopsis</i> centromeres

Popis výsledku v původním jazyce

Centromeres are critical for cell division, loading CENH3 or CENPA histone variant nucleosomes, directing kinetochore formation and allowing chromosome segregation(1,2). Despite their conserved function, centromere size and structure are diverse across species. To understand this centromere paradox(3,4), it is necessary to know how centromeric diversity is generated and whether it reflects ancient trans-species variation or, instead, rapid post-speciation divergence. To address these questions, we assembled 346 centromeres from 66 Arabidopsis thaliana and 2 Arabidopsis lyrata accessions, which exhibited a remarkable degree of intra- and inter-species diversity. A. thaliana centromere repeat arrays are embedded in linkage blocks, despite ongoing internal satellite turnover, consistent with roles for unidirectional gene conversion or unequal crossover between sister chromatids in sequence diversification. Additionally, centrophilic ATHILA transposons have recently invaded the satellite arrays. To counter ATHILA invasion, chromosome-specific bursts of satellite homogenization generate higher-order repeats and purge transposons, in line with cycles of repeat evolution. Centromeric sequence changes are even more extreme in comparison between A. thaliana and A. lyrata. Together, our findings identify rapid cycles of transposon invasion and purging through satellite homogenization, which drive centromere evolution and ultimately contribute to speciation.

Název v anglickém jazyce

Cycles of satellite and transposon evolution in <i>Arabidopsis</i> centromeres

Popis výsledku anglicky

Centromeres are critical for cell division, loading CENH3 or CENPA histone variant nucleosomes, directing kinetochore formation and allowing chromosome segregation(1,2). Despite their conserved function, centromere size and structure are diverse across species. To understand this centromere paradox(3,4), it is necessary to know how centromeric diversity is generated and whether it reflects ancient trans-species variation or, instead, rapid post-speciation divergence. To address these questions, we assembled 346 centromeres from 66 Arabidopsis thaliana and 2 Arabidopsis lyrata accessions, which exhibited a remarkable degree of intra- and inter-species diversity. A. thaliana centromere repeat arrays are embedded in linkage blocks, despite ongoing internal satellite turnover, consistent with roles for unidirectional gene conversion or unequal crossover between sister chromatids in sequence diversification. Additionally, centrophilic ATHILA transposons have recently invaded the satellite arrays. To counter ATHILA invasion, chromosome-specific bursts of satellite homogenization generate higher-order repeats and purge transposons, in line with cycles of repeat evolution. Centromeric sequence changes are even more extreme in comparison between A. thaliana and A. lyrata. Together, our findings identify rapid cycles of transposon invasion and purging through satellite homogenization, which drive centromere evolution and ultimately contribute to speciation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/GA21-03909S" target="_blank" >GA21-03909S: Odhalení evolučních tajů lničky seté a příbuzných druhů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature

ISSN

0028-0836

e-ISSN

1476-4687

Svazek periodika

618

Číslo periodika v rámci svazku

7965

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

34

Strana od-do

1-34

Kód UT WoS článku

000991386800003

EID výsledku v databázi Scopus

2-s2.0-85159477946