Visualising Enteroviruses 71 Genome Replication In Situ

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F24%3A00139214" target="_blank" >RIV/00216224:14740/24:00139214 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.jyu.fi/en/events/europic-2024?utm_source=chatgpt.com" target="_blank" >https://www.jyu.fi/en/events/europic-2024?utm_source=chatgpt.com</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Visualising Enteroviruses 71 Genome Replication In Situ

Popis výsledku v původním jazyce

Enterovirus 71 (EV71) is a member of the family Picornaviridae, non-enveloped +ssRNA viruses. EV71 is emerging as a significant causative agent of the hand-foot-and-mouth disease (HFMD), which is a prevalent viral illness predominantly afflicting small children. While HFMD typically presents with a skin rash, EV71 may also induce severe neurological complications, akin to polio. The life cycle of EV71 begins with the virus entering the cell via endocytosis, releasing its genome into the cytoplasm (1b). Within specialised viral factories, the viral genome undergoes replication. Simultaneously, the genome is translated. Thereafter, the assembly of new virions takes place. Upon lysis of the cell by the progeny viruses, the cycle restarts. Despite its significance, many aspects of this process remain poorly understood, making targeting the life cycle with antiviral drugs a persisting challenge. This project aims to investigate the lesser known aspects of EV71 life cycle - focusing on the genome replication process and its association with virion assembly. To enable this investigation, the development of a fluorescent reporter system to visualise the viral genome replication sites is presented here.

Název v anglickém jazyce

Visualising Enteroviruses 71 Genome Replication In Situ

Popis výsledku anglicky

Enterovirus 71 (EV71) is a member of the family Picornaviridae, non-enveloped +ssRNA viruses. EV71 is emerging as a significant causative agent of the hand-foot-and-mouth disease (HFMD), which is a prevalent viral illness predominantly afflicting small children. While HFMD typically presents with a skin rash, EV71 may also induce severe neurological complications, akin to polio. The life cycle of EV71 begins with the virus entering the cell via endocytosis, releasing its genome into the cytoplasm (1b). Within specialised viral factories, the viral genome undergoes replication. Simultaneously, the genome is translated. Thereafter, the assembly of new virions takes place. Upon lysis of the cell by the progeny viruses, the cycle restarts. Despite its significance, many aspects of this process remain poorly understood, making targeting the life cycle with antiviral drugs a persisting challenge. This project aims to investigate the lesser known aspects of EV71 life cycle - focusing on the genome replication process and its association with virion assembly. To enable this investigation, the development of a fluorescent reporter system to visualise the viral genome replication sites is presented here.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10607 - Virology

Projekt

<a href="/cs/project/EH22_008%2F0004607" target="_blank" >EH22_008/0004607: Nové technologie pro translační výzkum ve farmaceutických vědách /NETPHARM</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů