HAIRY ROOTS AS A POWERFUL TOOL TO STUDY HORMONAL AND ABIOTIC STRESS RESPONSE

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F25%3A00143963" target="_blank" >RIV/00216224:14740/25:00143963 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.recrop-cost.com/NewsAndActivities/ReadMore/8dd16317-8fc8-488e-dca1-08dddc48cd1e" target="_blank" >https://www.recrop-cost.com/NewsAndActivities/ReadMore/8dd16317-8fc8-488e-dca1-08dddc48cd1e</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

HAIRY ROOTS AS A POWERFUL TOOL TO STUDY HORMONAL AND ABIOTIC STRESS RESPONSE

Popis výsledku v původním jazyce

Hairy root cultures are a versatile system with many applications, including the production of recombinant proteins and valuable secondary metabolites, as well as the functional characterisation of genes. We have developed efficient transformation protocols for the spring cultivars of Brassica napus and Brassica rapa using hairy roots. We have generated various reporter lines, including new fluorescent reporters that monitor the response of the plant hormones auxin and cytokinin to abiotic stress stimuli. Furthermore, we have developed a pipeline for gene editing and hairy root regeneration in B. napus, with the aim of creating transgene-free, gene-edited plants. Since oilseed rape is an allotetraploid species (AACC, 2n=38), mutating homoeologs from both subgenomes is necessary to overcome functional genetic redundancy. Using optimised CRISPR/Cas9 vectors, we generated multiple mutant lines in four B. napus TRYPTOPHAN AMINOTRANSFERASE (BnaTAA1) auxin biosynthetic genes. In conclusion, we have initiated a functional analysis of BnaTAA1 and a study of the auxin biosynthesis pathway in oilseed rape using hairy root cultures. We offer transformation services for selected oilseed rape cultivars to generate CRISPR-edited plants and reporter lines. Funding sources: GA23-06140S by the Czech Science Foundation and TANGENC (TowArds Next GENeration Crops) CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius by MEYS.

Název v anglickém jazyce

HAIRY ROOTS AS A POWERFUL TOOL TO STUDY HORMONAL AND ABIOTIC STRESS RESPONSE

Popis výsledku anglicky

Hairy root cultures are a versatile system with many applications, including the production of recombinant proteins and valuable secondary metabolites, as well as the functional characterisation of genes. We have developed efficient transformation protocols for the spring cultivars of Brassica napus and Brassica rapa using hairy roots. We have generated various reporter lines, including new fluorescent reporters that monitor the response of the plant hormones auxin and cytokinin to abiotic stress stimuli. Furthermore, we have developed a pipeline for gene editing and hairy root regeneration in B. napus, with the aim of creating transgene-free, gene-edited plants. Since oilseed rape is an allotetraploid species (AACC, 2n=38), mutating homoeologs from both subgenomes is necessary to overcome functional genetic redundancy. Using optimised CRISPR/Cas9 vectors, we generated multiple mutant lines in four B. napus TRYPTOPHAN AMINOTRANSFERASE (BnaTAA1) auxin biosynthetic genes. In conclusion, we have initiated a functional analysis of BnaTAA1 and a study of the auxin biosynthesis pathway in oilseed rape using hairy root cultures. We offer transformation services for selected oilseed rape cultivars to generate CRISPR-edited plants and reporter lines. Funding sources: GA23-06140S by the Czech Science Foundation and TANGENC (TowArds Next GENeration Crops) CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius by MEYS.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10600 - Biological sciences

Projekt

<a href="/cs/project/EH22_008%2F0004581" target="_blank" >EH22_008/0004581: Nové poznatky pro plodiny nové generace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů