CRISPR-BASED GENOME EDITING IN BRASSICACEAE USING TISSUE CULTURE SYSTEMS
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F25%3A00143990" target="_blank" >RIV/00216224:14740/25:00143990 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.potravinyav21.cz/download/1826?utm_source=chatgpt.com" target="_blank" >https://www.potravinyav21.cz/download/1826?utm_source=chatgpt.com</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
CRISPR-BASED GENOME EDITING IN BRASSICACEAE USING TISSUE CULTURE SYSTEMS
Popis výsledku v původním jazyce
Hairy root cultures represent a versatile system applicable to a wide range of studies, including the functional characterization of genes and their regulatory elements. In our laboratory, we have established a CRISPR-based genome editing protocol for oilseed rape (Brassica napus) using hairy roots. This transformation system offers a straightforward and rapid approach to evaluate the functionality and efficiency of diverse CRISPR editing constructs. Using an optimized regeneration protocol, we are able to produce transgene-free, genome edited plant lines. Our future objectives involve the application of CRISPR technologies for base editing, prime editing, and transcriptional regulation within the Brassicaceae family using hairy root cultures.
Název v anglickém jazyce
CRISPR-BASED GENOME EDITING IN BRASSICACEAE USING TISSUE CULTURE SYSTEMS
Popis výsledku anglicky
Hairy root cultures represent a versatile system applicable to a wide range of studies, including the functional characterization of genes and their regulatory elements. In our laboratory, we have established a CRISPR-based genome editing protocol for oilseed rape (Brassica napus) using hairy roots. This transformation system offers a straightforward and rapid approach to evaluate the functionality and efficiency of diverse CRISPR editing constructs. Using an optimized regeneration protocol, we are able to produce transgene-free, genome edited plant lines. Our future objectives involve the application of CRISPR technologies for base editing, prime editing, and transcriptional regulation within the Brassicaceae family using hairy root cultures.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10611 - Plant sciences, botany
Návaznosti výsledku
Projekt
<a href="/cs/project/EH22_008%2F0004581" target="_blank" >EH22_008/0004581: Nové poznatky pro plodiny nové generace</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2025
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů