Je správné opomíjet fluorimetrické metody při stanovení glutathionu?

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Is it correct to leave out the fluorimetric methods for glutathione assay?

Popis výsledku v původním jazyce

Glutathione is the main intracellular antioxidant, which exists in two forms, the reduced and the oxidized. Changes in glutathione concentration serve as an important marker of the redox state inside the cells. Various methods can be used to measure glutathione concentration in biological material, namely enzymatic, fluorimetric or HPLC. Nevertheless, due to the high sensitivity and specificity HPLC methods are preferred to analyze glutathione concentration, in spite of high price and time demand. The aim of our study was to establish analytical parameters of the spectrofluorimetric method, which should be suitable for the analysis of huge amount of samples. This method is based on the well-known principle - reaction between glutathione and o phthaldialdehyde with subsequent production of strongly fluorescent isoindole-like product. To confirm selectivity of this method, we have analysed glutathione concentration in hepatocyte lysates using HPLC method with spectrofluorimetric detectio

Název v anglickém jazyce

Is it correct to leave out the fluorimetric methods for glutathione assay?

Popis výsledku anglicky

Glutathione is the main intracellular antioxidant, which exists in two forms, the reduced and the oxidized. Changes in glutathione concentration serve as an important marker of the redox state inside the cells. Various methods can be used to measure glutathione concentration in biological material, namely enzymatic, fluorimetric or HPLC. Nevertheless, due to the high sensitivity and specificity HPLC methods are preferred to analyze glutathione concentration, in spite of high price and time demand. The aim of our study was to establish analytical parameters of the spectrofluorimetric method, which should be suitable for the analysis of huge amount of samples. This method is based on the well-known principle - reaction between glutathione and o phthaldialdehyde with subsequent production of strongly fluorescent isoindole-like product. To confirm selectivity of this method, we have analysed glutathione concentration in hepatocyte lysates using HPLC method with spectrofluorimetric detectio

Druh

D - Stať ve sborníku

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Clin. Chim. Acta suppl.

ISBN

0009-8981

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

207-208

Název nakladatele

Elsevier

Místo vydání

UK

Místo konání akce

Glasgow, Skotsko

Datum konání akce

8. 5. 2005

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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