Employment of HPLC with coulometric detection for analysis of phytochelatin synthase activity

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Employment of HPLC with coulometric detection for analysis of phytochelatin synthase activity

Popis výsledku v původním jazyce

Phytochelatins are physiologically active peptides which have to be synthesized by phytochelatin synthase (PCS). The main aim of this study was to optimize high performance liquid chromatography coupled with electrochemical detector (HPLC-ED) as suitabletool for determination of the phytochelatin synthase activity. Coulometric detector with porous graphite working electrode was used. Firstly we optimized a separation of two compounds which are important for monitoring of PCS activity. It was glutathione reduced as substrate for the reaction and fytochelatin-2 as a product. PCS is the best activated by cadmium ions. We used a model with BY-2 tobacco cells. We conducted the in vivo 3 day cultivation experiment where BY-2 cells were treated by various concentrations of cadmium. We then homogenized the cells and immediately analyzed the extracts by optimized HPLC-ED method. Moreover we observed that with higher concentrations of applied cadmium there was increasing of amount of PC-2. The

Název v anglickém jazyce

Employment of HPLC with coulometric detection for analysis of phytochelatin synthase activity

Popis výsledku anglicky

Phytochelatins are physiologically active peptides which have to be synthesized by phytochelatin synthase (PCS). The main aim of this study was to optimize high performance liquid chromatography coupled with electrochemical detector (HPLC-ED) as suitabletool for determination of the phytochelatin synthase activity. Coulometric detector with porous graphite working electrode was used. Firstly we optimized a separation of two compounds which are important for monitoring of PCS activity. It was glutathione reduced as substrate for the reaction and fytochelatin-2 as a product. PCS is the best activated by cadmium ions. We used a model with BY-2 tobacco cells. We conducted the in vivo 3 day cultivation experiment where BY-2 cells were treated by various concentrations of cadmium. We then homogenized the cells and immediately analyzed the extracts by optimized HPLC-ED method. Moreover we observed that with higher concentrations of applied cadmium there was increasing of amount of PC-2. The

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

XI. pracovní setkání fyzikálních chemiků a elektrochemiků

ISBN

978-80-7375-514-0

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

332-335

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

MENDELU

Datum konání akce

1. 1. 2011

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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