Phytochelatin synthase activity as a marker of metal pollution
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F11%3A43870544" target="_blank" >RIV/62157124:16270/11:43870544 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62156489:43210/11:00176233 RIV/00216305:26620/11:PU95050
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.jhazmat.2011.05.088" target="_blank" >http://dx.doi.org/10.1016/j.jhazmat.2011.05.088</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jhazmat.2011.05.088" target="_blank" >10.1016/j.jhazmat.2011.05.088</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Phytochelatin synthase activity as a marker of metal pollution
Popis výsledku v původním jazyce
The synthesis of phytochelatins is catalyzed by gama-Glu-Cys dipeptidyl transpeptidase called phytochelatin synthase (PCS). Aim of this study was to suggest a new tool for determination of phytochelatin synthase activity in the tobacco BY-2 cells treatedwith different concentrations of the Cd(II). After the optimization steps, an experiment on BY-2 cells exposed to different concentrations of Cd(NO(3))(2) for 3 days was performed. At the end of the experiment, cells were harvested and homogenized. Reduced glutathione and cadmium (II) ions were added to the cell suspension supernatant. These mixtures were incubated at 35°C for 30min and analysed using high performance liquid chromatography coupled with electrochemical detector (HPLC-ED). The results revealed that PCS activity rises markedly with increasing concentration of cadmium (II) ions. The lowest concentration of the toxic metal ions caused almost three fold increase in PCS activity as compared to control samples. The activity of
Název v anglickém jazyce
Phytochelatin synthase activity as a marker of metal pollution
Popis výsledku anglicky
The synthesis of phytochelatins is catalyzed by gama-Glu-Cys dipeptidyl transpeptidase called phytochelatin synthase (PCS). Aim of this study was to suggest a new tool for determination of phytochelatin synthase activity in the tobacco BY-2 cells treatedwith different concentrations of the Cd(II). After the optimization steps, an experiment on BY-2 cells exposed to different concentrations of Cd(NO(3))(2) for 3 days was performed. At the end of the experiment, cells were harvested and homogenized. Reduced glutathione and cadmium (II) ions were added to the cell suspension supernatant. These mixtures were incubated at 35°C for 30min and analysed using high performance liquid chromatography coupled with electrochemical detector (HPLC-ED). The results revealed that PCS activity rises markedly with increasing concentration of cadmium (II) ions. The lowest concentration of the toxic metal ions caused almost three fold increase in PCS activity as compared to control samples. The activity of
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Hazardous Materials
ISSN
0304-3894
e-ISSN
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Svazek periodika
192
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
7
Strana od-do
794-800
Kód UT WoS článku
000293314000048
EID výsledku v databázi Scopus
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