A novel method for analysis of plants' resistance to heavy metals

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F11%3A00180218" target="_blank" >RIV/62156489:43210/11:00180218 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2011/mendelnet_2011_fulltext.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2011/mendelnet_2011_fulltext.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A novel method for analysis of plants' resistance to heavy metals

Popis výsledku v původním jazyce

Nowadays many less developed countries has many problems concerning about pollution in environment caused by heavy metals as cadmium, lead or mercury. The main aim of this work was to optimize method for determination of activity of main plant protectivemechanism against the heavy metals. Sulphur rich peptides phytochlatins (PC's) are physiologically active compounds because they are able of immobilization of the toxic heavy metal directly in the plant. Most occurring of PC's is phytochelatin2 (PC-2) which is synthesized by phytochelatin synthase (PCS). We focused on developing of the method based on high performance liquid chromatography coupled with electrochemical detector (HPLC-ED) for determination of PC-2 because it might serve as suitable toolfor determination of phytochelatin synthase activity. PCS is the best activated by cadmium ions. We used a model with BY-2 tobacco cells. We conducted the in vivo 3 day cultivation experiment where BY-2 cells were treated by various conce

Název v anglickém jazyce

A novel method for analysis of plants' resistance to heavy metals

Popis výsledku anglicky

Nowadays many less developed countries has many problems concerning about pollution in environment caused by heavy metals as cadmium, lead or mercury. The main aim of this work was to optimize method for determination of activity of main plant protectivemechanism against the heavy metals. Sulphur rich peptides phytochlatins (PC's) are physiologically active compounds because they are able of immobilization of the toxic heavy metal directly in the plant. Most occurring of PC's is phytochelatin2 (PC-2) which is synthesized by phytochelatin synthase (PCS). We focused on developing of the method based on high performance liquid chromatography coupled with electrochemical detector (HPLC-ED) for determination of PC-2 because it might serve as suitable toolfor determination of phytochelatin synthase activity. PCS is the best activated by cadmium ions. We used a model with BY-2 tobacco cells. We conducted the in vivo 3 day cultivation experiment where BY-2 cells were treated by various conce

Druh

D - Stať ve sborníku

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2011

ISBN

978-80-7375-563-8

ISSN

—

e-ISSN

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Počet stran výsledku

5

Strana od-do

1060-1064

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

1. 1. 2011

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

341783400130