Stanovení beta-karotenu v lidské plazmě pomocí HPLC s UV detekcí

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F07%3A00006169" target="_blank" >RIV/00216275:25310/07:00006169 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of beta-carotene in human plasma by HPLC with ultraviolet detection

Popis výsledku v původním jazyce

Beta-carotene plays an important role in biological systems. It protects against the action of some reactive oxygen and nitrogen species. A method for determination of beta-carotene in human plasma has been developed and evaluated. Chromatography was accomplished using isocratic elution on LiChrocart 125-4 Purospher RP-18e, 5 um, column. The mixture of methanol and ethanol (75:25, v/v) was used as a mobile phase. As internal standard was chosen retinyl palmitate. The analytical performance of this method is satisfactory, both intra-assay and inter-assay were with coefficients of variation below 10%, recovery was between 90-110%. The calibration curve was linear in the whole range tested (0.02-2.00) umol/L. The detection limit was 0.5 pmol/20 ul inject.This assay is a suitable and reproducible HPLC method for the determination of beta-carotene in human plasma. Acknowledgments. This work was supported by grant MSM0021627502.

Název v anglickém jazyce

Determination of beta-carotene in human plasma by HPLC with ultraviolet detection

Popis výsledku anglicky

Beta-carotene plays an important role in biological systems. It protects against the action of some reactive oxygen and nitrogen species. A method for determination of beta-carotene in human plasma has been developed and evaluated. Chromatography was accomplished using isocratic elution on LiChrocart 125-4 Purospher RP-18e, 5 um, column. The mixture of methanol and ethanol (75:25, v/v) was used as a mobile phase. As internal standard was chosen retinyl palmitate. The analytical performance of this method is satisfactory, both intra-assay and inter-assay were with coefficients of variation below 10%, recovery was between 90-110%. The calibration curve was linear in the whole range tested (0.02-2.00) umol/L. The detection limit was 0.5 pmol/20 ul inject.This assay is a suitable and reproducible HPLC method for the determination of beta-carotene in human plasma. Acknowledgments. This work was supported by grant MSM0021627502.

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Vitamins 2007 - Nutrition and Diagnostics

ISBN

978-80-7194-937-4

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

216

Název nakladatele

Univerzita Pardubice

Místo vydání

Pardubice

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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