Stanovení redukovaného a oxidovaného glutathionu v biologickém materiálu pomocí HPLC s elektrochemickou detekcí

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of reduced and oxidized glutathione in biological material by HPLC with electrochemical detection

Popis výsledku v původním jazyce

Background. Tripeptid glutathione plays an important role in biological systems. It protects against the action of some reactive oxygen and nitrogen species and is involved in detoxication of harmful compounds. Methods. A method for determination of bothreduced and oxidized glutathione in whole blood and hepatocytes has been developed and evaluated. Chromatography of both reduced and oxidized glutathione was accomplished using isocratic elution on Polaris 5u C18-A, 250 x 4.6 mm, i.d. column. The mixture of methanol and 25 mmol/L sodium dihydrogen phosphate, pH 3.0 (6:94 v/v) was used as a mobile phase. Results. The analytical performance of this method is satisfactory. The intra-assay coefficients of variation in whole blood and rat hepatocytes were 2.1% and 2.2% for reduced and 4.7% and 4.9% for oxidized glutathione. The recoveries were following: 91.2% (CV 3.3%) for reduced and 94.1% (CV 8.3%) for oxidized glutathione in the whole blood. The calibration curve was linear in the whole

Název v anglickém jazyce

Determination of reduced and oxidized glutathione in biological material by HPLC with electrochemical detection

Popis výsledku anglicky

Background. Tripeptid glutathione plays an important role in biological systems. It protects against the action of some reactive oxygen and nitrogen species and is involved in detoxication of harmful compounds. Methods. A method for determination of bothreduced and oxidized glutathione in whole blood and hepatocytes has been developed and evaluated. Chromatography of both reduced and oxidized glutathione was accomplished using isocratic elution on Polaris 5u C18-A, 250 x 4.6 mm, i.d. column. The mixture of methanol and 25 mmol/L sodium dihydrogen phosphate, pH 3.0 (6:94 v/v) was used as a mobile phase. Results. The analytical performance of this method is satisfactory. The intra-assay coefficients of variation in whole blood and rat hepatocytes were 2.1% and 2.2% for reduced and 4.7% and 4.9% for oxidized glutathione. The recoveries were following: 91.2% (CV 3.3%) for reduced and 94.1% (CV 8.3%) for oxidized glutathione in the whole blood. The calibration curve was linear in the whole

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Clinical Chemistry and Laboratory Medicine

ISBN

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ISSN

1434-6621

e-ISSN

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Počet stran výsledku

1

Strana od-do

"S195"

Název nakladatele

Walter de Gruyter GmbH & Co. KG

Místo vydání

Berlin

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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