Gradient HPLC: Gradient System Selection

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Gradient HPLC: Gradient System Selection

Popis výsledku v původním jazyce

Gradient elution is widely used for the separation of complex samples in reversed-phase high-performance liquid chromatography systems and ion-exchange systems. The theory of gradient elution has now been elaborated so that it allows predicting the retention and the resolution of sample compounds in reversed-phase, ion-exchange, and normal-phase systems for almost any combination of gradient profile and relationship between the retention and mobile-phase composition. The most important sources of errorsin the prediction and optimization of gradient elution are the gradient dwell volume and the preferential adsorption of the strong solvents, especially in normal-phase (adsorption) chromatography. The gradient dwell volume effects are more important inmicro-LC techniques than in conventional LC and may cause significant increase in the time of analysis, unless special instrumentation and (or) pre-column flow splitting is used, but their effects can be taken into account in predictive c

Název v anglickém jazyce

Gradient HPLC: Gradient System Selection

Popis výsledku anglicky

Gradient elution is widely used for the separation of complex samples in reversed-phase high-performance liquid chromatography systems and ion-exchange systems. The theory of gradient elution has now been elaborated so that it allows predicting the retention and the resolution of sample compounds in reversed-phase, ion-exchange, and normal-phase systems for almost any combination of gradient profile and relationship between the retention and mobile-phase composition. The most important sources of errorsin the prediction and optimization of gradient elution are the gradient dwell volume and the preferential adsorption of the strong solvents, especially in normal-phase (adsorption) chromatography. The gradient dwell volume effects are more important inmicro-LC techniques than in conventional LC and may cause significant increase in the time of analysis, unless special instrumentation and (or) pre-column flow splitting is used, but their effects can be taken into account in predictive c

Druh

C - Kapitola v odborné knize

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Encyclopedia of Chromatography

ISBN

978-1-4200-8459-7

Počet stran výsledku

13

Strana od-do

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Počet stran knihy

2850

Název nakladatele

CRC Press

Místo vydání

London

Kód UT WoS kapitoly

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