Possibilities of Retention Prediction in Fast Gradient Liquid Chromatography. Part 1: Comparison of Separation on Packed Fully Porous, Nonporous and Monolithic Columns

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896312" target="_blank" >RIV/00216275:25310/13:39896312 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0021967313000228" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0021967313000228</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2012.12.058" target="_blank" >10.1016/j.chroma.2012.12.058</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Possibilities of Retention Prediction in Fast Gradient Liquid Chromatography. Part 1: Comparison of Separation on Packed Fully Porous, Nonporous and Monolithic Columns

Popis výsledku v původním jazyce

Due to ever increasing importance of short analysis times in modern HPLC practice, fast gradient elution is becoming widely used both in one-dimensional and two-dimensional separations, especially in the second dimension where the speed of separation isof primary importance. For method development and optimization, prediction of retention in gradient elution from the isocratic data is very important. This is principally possible using the well-established theory of gradient elution, especially for reversed-phase separations. Feasibility of these approaches has been proven in conventional liquid chromatography and commercial prediction software is available for this purpose. However, fast gradient chromatography employing short columns and gradient times in between 1 and 2 min or even less, impose additional stringent demands on the accuracy of prediction, due to rapid changes in mobile phase composition and increased role of extra-column contributions and instrumental dwell volume on

Název v anglickém jazyce

Possibilities of Retention Prediction in Fast Gradient Liquid Chromatography. Part 1: Comparison of Separation on Packed Fully Porous, Nonporous and Monolithic Columns

Popis výsledku anglicky

Due to ever increasing importance of short analysis times in modern HPLC practice, fast gradient elution is becoming widely used both in one-dimensional and two-dimensional separations, especially in the second dimension where the speed of separation isof primary importance. For method development and optimization, prediction of retention in gradient elution from the isocratic data is very important. This is principally possible using the well-established theory of gradient elution, especially for reversed-phase separations. Feasibility of these approaches has been proven in conventional liquid chromatography and commercial prediction software is available for this purpose. However, fast gradient chromatography employing short columns and gradient times in between 1 and 2 min or even less, impose additional stringent demands on the accuracy of prediction, due to rapid changes in mobile phase composition and increased role of extra-column contributions and instrumental dwell volume on

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GAP206%2F12%2F0398" target="_blank" >GAP206/12/0398: Dvourozměrné separace v kapalných médiích</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1278

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

37-45

Kód UT WoS článku

000315011200006

EID výsledku v databázi Scopus

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