Isocratic HPLC: System Selection

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isocratic HPLC: System Selection

Popis výsledku v původním jazyce

The effects of various factors on the separation in isocratic HPLC are discussed. Various separation modes, including aqueous-organic and non-aqueous reversedphase (RP) chromatography, adsorption normal-phase liquid chromatography (NPLC), hydrophilic interaction liquid chromatography (HILIC), ion-exchange, ion-pair, and size-exclusion chromatography (SEC) for non-ionic samples differing in the size and polarities and for partially or fully ionized acidic or basic compounds are addressed. Selection of the column length, inner diameter (I.D.), character of the column bed, and the type of stationary phase are discussed, using the information on the sample properties, such as solubility, polarity, presence of specific functional groups in sample compounds,sample amount and concentration, and so on. Empirical and model-based approaches to the optimization of the operation parameters controlling the separation selectivity, efficiency, and resolution in HPLC are outlined.

Název v anglickém jazyce

Isocratic HPLC: System Selection

Popis výsledku anglicky

The effects of various factors on the separation in isocratic HPLC are discussed. Various separation modes, including aqueous-organic and non-aqueous reversedphase (RP) chromatography, adsorption normal-phase liquid chromatography (NPLC), hydrophilic interaction liquid chromatography (HILIC), ion-exchange, ion-pair, and size-exclusion chromatography (SEC) for non-ionic samples differing in the size and polarities and for partially or fully ionized acidic or basic compounds are addressed. Selection of the column length, inner diameter (I.D.), character of the column bed, and the type of stationary phase are discussed, using the information on the sample properties, such as solubility, polarity, presence of specific functional groups in sample compounds,sample amount and concentration, and so on. Empirical and model-based approaches to the optimization of the operation parameters controlling the separation selectivity, efficiency, and resolution in HPLC are outlined.

Druh

C - Kapitola v odborné knize

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Encyclopedia of Chromatography

ISBN

978-1-4200-8459-7

Počet stran výsledku

11

Strana od-do

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Počet stran knihy

2850

Název nakladatele

CRC Press

Místo vydání

London

Kód UT WoS kapitoly

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