Comparison of various modes and phase systems for analytical HPLC

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F20%3A39916970" target="_blank" >RIV/00216275:25310/20:39916970 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/B978-0-444-64070-3.00001-1" target="_blank" >http://dx.doi.org/10.1016/B978-0-444-64070-3.00001-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/B978-0-444-64070-3.00001-1" target="_blank" >10.1016/B978-0-444-64070-3.00001-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of various modes and phase systems for analytical HPLC

Popis výsledku v původním jazyce

This chapter addresses principles of the separation modes used in contemporary column liquid chromatography, including reversed-phase organic normal-phase hydrophilic interaction liquid chromatography (aqueous normal-phase chromatography) and ion-exchange separation systems, with respect to the effects of the stationary and mobile phases and the experimental conditions on the separation. This treatment compares the advantages and disadvantages of various types and sizes of column particles, column formats and dimensions on the efficiency of separation, with special attention to the sub-2 μm particles, core-shell and monolithic columns. Theoretical retention models for the individual separation modes deal with the selection of the mobile phase in the isocratic retention models and possible extension to gradient elution separations. Temperature affects the sample diffusivity and hence the separation efficiency on one side, and the thermodynamic contributions of enthalpy and entropy to the energy of retention on the other. Serial, heart-cutting and comprehensive two-dimensional techniques combining independent (orthogonal) separation modes largely increase the peak capacity of liquid chromatography.

Název v anglickém jazyce

Comparison of various modes and phase systems for analytical HPLC

Popis výsledku anglicky

This chapter addresses principles of the separation modes used in contemporary column liquid chromatography, including reversed-phase organic normal-phase hydrophilic interaction liquid chromatography (aqueous normal-phase chromatography) and ion-exchange separation systems, with respect to the effects of the stationary and mobile phases and the experimental conditions on the separation. This treatment compares the advantages and disadvantages of various types and sizes of column particles, column formats and dimensions on the efficiency of separation, with special attention to the sub-2 μm particles, core-shell and monolithic columns. Theoretical retention models for the individual separation modes deal with the selection of the mobile phase in the isocratic retention models and possible extension to gradient elution separations. Temperature affects the sample diffusivity and hence the separation efficiency on one side, and the thermodynamic contributions of enthalpy and entropy to the energy of retention on the other. Serial, heart-cutting and comprehensive two-dimensional techniques combining independent (orthogonal) separation modes largely increase the peak capacity of liquid chromatography.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.

Název knihy nebo sborníku

Handbook of Analytical Separations. Vol. 8

ISBN

978-0-444-64070-3

Počet stran výsledku

91

Strana od-do

1-91

Počet stran knihy

788

Název nakladatele

Elsevier Science BV

Místo vydání

Amsterdam

Kód UT WoS kapitoly

—