Identification of Phase I and Phase II Metabolites of Benfluron and Dimefluron in Rat Urine Using High-performance Liquid Chromatography /Tandem Mass Spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892077" target="_blank" >RIV/00216275:25310/11:39892077 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/11:10100618

Výsledek na webu

<a href="http://dx.doi.org/10.1002/rcm.5097" target="_blank" >http://dx.doi.org/10.1002/rcm.5097</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/rcm.5097" target="_blank" >10.1002/rcm.5097</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of Phase I and Phase II Metabolites of Benfluron and Dimefluron in Rat Urine Using High-performance Liquid Chromatography /Tandem Mass Spectrometry

Popis výsledku v původním jazyce

Biotransformation products of two potential antineoplastic agents are characterized using HPLC/UV/MS/MS. High mass accuracy measurement allows confirmation of an elemental composition and metabolic reactions according to exact mass defects. The combination of different HPLC/MS/MS scans, such as reconstructed ion current chromatograms, constant neutral loss chromatograms or exact mass filtration, helps the unambiguous detection of low abundance metabolites. The arene oxidation, N-oxidation, N-demethylation, O-demethylation, carbonyl reduction, glucuronidation and sulfation are typical mechanisms of the metabolite formation. The interpretation of their tandem mass spectra enables the distinction of demethylation position (N- vs. O-) as well as to differentiate N-oxidation from arene oxidation for both phase I and phase II metabolites. Two metabolic pathways are rather unusual for rat samples, i.e., glucosylation and double glucuronidation.

Název v anglickém jazyce

Identification of Phase I and Phase II Metabolites of Benfluron and Dimefluron in Rat Urine Using High-performance Liquid Chromatography /Tandem Mass Spectrometry

Popis výsledku anglicky

Biotransformation products of two potential antineoplastic agents are characterized using HPLC/UV/MS/MS. High mass accuracy measurement allows confirmation of an elemental composition and metabolic reactions according to exact mass defects. The combination of different HPLC/MS/MS scans, such as reconstructed ion current chromatograms, constant neutral loss chromatograms or exact mass filtration, helps the unambiguous detection of low abundance metabolites. The arene oxidation, N-oxidation, N-demethylation, O-demethylation, carbonyl reduction, glucuronidation and sulfation are typical mechanisms of the metabolite formation. The interpretation of their tandem mass spectra enables the distinction of demethylation position (N- vs. O-) as well as to differentiate N-oxidation from arene oxidation for both phase I and phase II metabolites. Two metabolic pathways are rather unusual for rat samples, i.e., glucosylation and double glucuronidation.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Rapid Communication in Mass Spectrometry

ISSN

0951-4198

e-ISSN

—

Svazek periodika

25

Číslo periodika v rámci svazku

—

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

2153-2162

Kód UT WoS článku

000292552800006

EID výsledku v databázi Scopus

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