Identification of new phase II metabolites of xanthohumol in rat in vivo biotransformation of hop extracts using high-performance liquid chromatography electrospray ionization tandem mass spectrometry
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F10%3A10213188" target="_blank" >RIV/61989592:15110/10:10213188 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216275:25310/10:39881357
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Identification of new phase II metabolites of xanthohumol in rat in vivo biotransformation of hop extracts using high-performance liquid chromatography electrospray ionization tandem mass spectrometry
Popis výsledku v původním jazyce
Polyphenolic compounds occurring in hop extracts and their phase I and II metabolites formed during in vivo rat biotransformation have been analyzed using HPLC/MS/MS. In our study, hybrid quadrupole - time-of-flight analyzer is used for the identification of both natural phenolics and their metabolites due to the possibility of accurate mass easurements in full scan and tandem mass spectra supported by MSn data obtained with the ion trap analyzer. The emphasis is given on the elemental composition determination of individual metabolites based on accurate masses typically better than 5 ppm even with the external calibration. Chromatograms of rat incubations are also compared with chromatograms of pure rat feed, rat feed enriched with hop extracts and the placebo experiment. More than ten compounds originating from the hops are identified in rat feces, two of them belong to phase I metabolites and five compounds are phase II metabolites.
Název v anglickém jazyce
Identification of new phase II metabolites of xanthohumol in rat in vivo biotransformation of hop extracts using high-performance liquid chromatography electrospray ionization tandem mass spectrometry
Popis výsledku anglicky
Polyphenolic compounds occurring in hop extracts and their phase I and II metabolites formed during in vivo rat biotransformation have been analyzed using HPLC/MS/MS. In our study, hybrid quadrupole - time-of-flight analyzer is used for the identification of both natural phenolics and their metabolites due to the possibility of accurate mass easurements in full scan and tandem mass spectra supported by MSn data obtained with the ion trap analyzer. The emphasis is given on the elemental composition determination of individual metabolites based on accurate masses typically better than 5 ppm even with the external calibration. Chromatograms of rat incubations are also compared with chromatograms of pure rat feed, rat feed enriched with hop extracts and the placebo experiment. More than ten compounds originating from the hops are identified in rat feces, two of them belong to phase I metabolites and five compounds are phase II metabolites.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GA203%2F08%2F1536" target="_blank" >GA203/08/1536: Progresívní metody v chemické, potravinářské a klinické analýze</a><br>
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2010
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Chromatography A
ISSN
0021-9673
e-ISSN
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Svazek periodika
25
Číslo periodika v rámci svazku
1217
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
9
Strana od-do
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Kód UT WoS článku
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EID výsledku v databázi Scopus
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