Determination of serum creatinine in selected groups of patients by means of HPLC with UV detection - comparison with Jaffé and enzymatic assays

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892308" target="_blank" >RIV/00216275:25310/11:39892308 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of serum creatinine in selected groups of patients by means of HPLC with UV detection - comparison with Jaffé and enzymatic assays

Popis výsledku v původním jazyce

Results from measurements of by a high-performance liquid chromatography on 232 serum samples were compared with enzymatic and Jaffé methods. The serum samples were deproteinized with ethanol. For the separation, a column MAG 1, 250 mm x 4.6 mm, Labiospher PSI 100 C18, 5 mým, was used. The mixture of ethanol and 25 mmol l-1 sodium hydrogenphosphate (3:97 v/v), pH 6.5 was used as a mobile phase. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 6 %. Quantitative recoveries of spiked serum samples were between 101.8 and 106.0 %. The limit of quantification was 10.0 mýmol l-1. The results obtained by chromatographic method correlated well with Jaffé assays. Enzymatic method gaveat average significantly higher values depending on a group of patients.

Název v anglickém jazyce

Determination of serum creatinine in selected groups of patients by means of HPLC with UV detection - comparison with Jaffé and enzymatic assays

Popis výsledku anglicky

Results from measurements of by a high-performance liquid chromatography on 232 serum samples were compared with enzymatic and Jaffé methods. The serum samples were deproteinized with ethanol. For the separation, a column MAG 1, 250 mm x 4.6 mm, Labiospher PSI 100 C18, 5 mým, was used. The mixture of ethanol and 25 mmol l-1 sodium hydrogenphosphate (3:97 v/v), pH 6.5 was used as a mobile phase. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 6 %. Quantitative recoveries of spiked serum samples were between 101.8 and 106.0 %. The limit of quantification was 10.0 mýmol l-1. The results obtained by chromatographic method correlated well with Jaffé assays. Enzymatic method gaveat average significantly higher values depending on a group of patients.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology

ISSN

1211-5541

e-ISSN

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Svazek periodika

17

Číslo periodika v rámci svazku

2011

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

14

Strana od-do

57-70

Kód UT WoS článku

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EID výsledku v databázi Scopus

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