A New Biosensor for Glucose Based on Carbon Paste and Enzyme Immobilized onto the Polyaniline Film

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892617" target="_blank" >RIV/00216275:25310/11:39892617 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A New Biosensor for Glucose Based on Carbon Paste and Enzyme Immobilized onto the Polyaniline Film

Popis výsledku v původním jazyce

Conductive polymer polyaniline (PANI) was electrochemically synthesized by controlled potential cycling as a film at the surface of a carbon paste electrode (CPE) from acidic aqueous solution containing 1 M HCl and 0.25 M aniline. The characterization ofthe PANI-CPE was carried out with the aid of cyclic voltammetry in 1 M HCl, obtaining three couple of peaks from which the two pairs were distinct, having proved the electroactivity of the PANI film. Then, the PANI-CPE system was for the first time usedand tested as glucose biosensor, when the respective enzyme was potentiostatically immobilized onto the surface from 0.1 M phosphate buffer (pH 7.5) with 0.75 mg/mL GOD. Optimal conditions for the enzyme immobilization were investigated and the biosensor tested for glucose sensing within the concentration range of 1-500 mg/L D-glucose. All preliminary assays with the GOD/PANI-CPE configuration have shown favorable operational features of this new carbon paste biosensor whose further dev

Název v anglickém jazyce

A New Biosensor for Glucose Based on Carbon Paste and Enzyme Immobilized onto the Polyaniline Film

Popis výsledku anglicky

Conductive polymer polyaniline (PANI) was electrochemically synthesized by controlled potential cycling as a film at the surface of a carbon paste electrode (CPE) from acidic aqueous solution containing 1 M HCl and 0.25 M aniline. The characterization ofthe PANI-CPE was carried out with the aid of cyclic voltammetry in 1 M HCl, obtaining three couple of peaks from which the two pairs were distinct, having proved the electroactivity of the PANI film. Then, the PANI-CPE system was for the first time usedand tested as glucose biosensor, when the respective enzyme was potentiostatically immobilized onto the surface from 0.1 M phosphate buffer (pH 7.5) with 0.75 mg/mL GOD. Optimal conditions for the enzyme immobilization were investigated and the biosensor tested for glucose sensing within the concentration range of 1-500 mg/L D-glucose. All preliminary assays with the GOD/PANI-CPE configuration have shown favorable operational features of this new carbon paste biosensor whose further dev

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

<a href="/cs/project/LC06035" target="_blank" >LC06035: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CS - Srbsko a Černá Hora

Počet stran výsledku

10

Strana od-do

1917-1926

Kód UT WoS článku

000292330700014

EID výsledku v databázi Scopus

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