Non-Targeted Quantitation of Lipid Classes Using Hydrophilic Interaction Liquid Chromatography - Electrospray Ionization Mass Spectrometry Using Single Internal Standard and Response Factor Approach
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F12%3A39895379" target="_blank" >RIV/00216275:25310/12:39895379 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1021/ac3024476" target="_blank" >http://dx.doi.org/10.1021/ac3024476</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/ac3024476" target="_blank" >10.1021/ac3024476</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Non-Targeted Quantitation of Lipid Classes Using Hydrophilic Interaction Liquid Chromatography - Electrospray Ionization Mass Spectrometry Using Single Internal Standard and Response Factor Approach
Popis výsledku v původním jazyce
The identification and quantitation of a wide range of lipids in complex biological samples is an essential requirement for the lipidomic studies. High-performance liquid chromatography?mass spectrometry (HPLC/MS) has the highest potential to obtain detailed information on the whole lipidome, but the reliable quantitation of multiple lipid classes is still a challenging task. In this work, we describe a new method for the nontargeted quantitation of polar lipid classes separated by hydrophilic interaction liquid chromatography (HILIC) followed by positive-ion electrospray ionization mass spectrometry (ESI-MS) using a single internal lipid standard to which all class specific response factors (RFs) are related to. The developed method enables the nontargeted quantitation of lipid classes and molecules inside these classes in contrast to the conventional targeted quantitation, which is based on predefined selected reaction monitoring (SRM) transitions for selected lipids only. In the non
Název v anglickém jazyce
Non-Targeted Quantitation of Lipid Classes Using Hydrophilic Interaction Liquid Chromatography - Electrospray Ionization Mass Spectrometry Using Single Internal Standard and Response Factor Approach
Popis výsledku anglicky
The identification and quantitation of a wide range of lipids in complex biological samples is an essential requirement for the lipidomic studies. High-performance liquid chromatography?mass spectrometry (HPLC/MS) has the highest potential to obtain detailed information on the whole lipidome, but the reliable quantitation of multiple lipid classes is still a challenging task. In this work, we describe a new method for the nontargeted quantitation of polar lipid classes separated by hydrophilic interaction liquid chromatography (HILIC) followed by positive-ion electrospray ionization mass spectrometry (ESI-MS) using a single internal lipid standard to which all class specific response factors (RFs) are related to. The developed method enables the nontargeted quantitation of lipid classes and molecules inside these classes in contrast to the conventional targeted quantitation, which is based on predefined selected reaction monitoring (SRM) transitions for selected lipids only. In the non
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GAP206%2F11%2F0022" target="_blank" >GAP206/11/0022: Lipidomický profil pacientů s kardiovaskulárními chorobami</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Analytical Chemistry
ISSN
0003-2700
e-ISSN
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Svazek periodika
84
Číslo periodika v rámci svazku
22
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
7
Strana od-do
10064-10070
Kód UT WoS článku
000312434800056
EID výsledku v databázi Scopus
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