New lipidomics approach: High-throughput nontargeted lipidomic analysis using supercritical fluid chromatography/ion-mobility mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39899335" target="_blank" >RIV/00216275:25310/14:39899335 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

New lipidomics approach: High-throughput nontargeted lipidomic analysis using supercritical fluid chromatography/ion-mobility mass spectrometry

Popis výsledku v původním jazyce

Lipidomic analysis is challenging due to enormous complexity of lipid structures. Two well-established analytical approaches are commonly used in lipidomics, such as shotgun ESI-MS/MS and LC/MS, both having some advantages and limitations. Alternative high-throughput and nontargeted approach is developed in this work using supercritical fluid chromatography (SFC) followed by ESI and ion mobility-mass spectrometry (IM-MS) for the determination of lipid composition. Individual parameters of SFC analysis have been carefully optimized to achieve a maximum number of separated lipid classes. Developed SFC method enables the separation of 26 nonpolar and polar lipid classes within 6 minutes. Mass spectra with high mass accuracy and resolving power are acquired using ESI in both positive- and negative-ion modes for the identification and quantitation of individual species. The quantitative analysis is performed using internal standards for each lipid class. IM is used as an additional separati

Název v anglickém jazyce

New lipidomics approach: High-throughput nontargeted lipidomic analysis using supercritical fluid chromatography/ion-mobility mass spectrometry

Popis výsledku anglicky

Lipidomic analysis is challenging due to enormous complexity of lipid structures. Two well-established analytical approaches are commonly used in lipidomics, such as shotgun ESI-MS/MS and LC/MS, both having some advantages and limitations. Alternative high-throughput and nontargeted approach is developed in this work using supercritical fluid chromatography (SFC) followed by ESI and ion mobility-mass spectrometry (IM-MS) for the determination of lipid composition. Individual parameters of SFC analysis have been carefully optimized to achieve a maximum number of separated lipid classes. Developed SFC method enables the separation of 26 nonpolar and polar lipid classes within 6 minutes. Mass spectra with high mass accuracy and resolving power are acquired using ESI in both positive- and negative-ion modes for the identification and quantitation of individual species. The quantitative analysis is performed using internal standards for each lipid class. IM is used as an additional separati

Druh

O - Ostatní výsledky

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/LL1302" target="_blank" >LL1302: Hmotnostní spektrometrie při hledání lipidových biomarkerů pro včasnou diagnostiku rakoviny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů