Lipidomics: Supercritical fluid chromatography/ion mobility - mass spectrometry as a tool for fast nontargeted analysis of lipids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39899334" target="_blank" >RIV/00216275:25310/14:39899334 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Lipidomics: Supercritical fluid chromatography/ion mobility - mass spectrometry as a tool for fast nontargeted analysis of lipids

Popis výsledku v původním jazyce

Individual parameters of SFC analysis have been carefully optimized to achieve a maximum number of separated lipid classes. Developed SFC method enables the fast separation of 16 nonpolar and polar lipid classes within 6 minute analysis including the partial separation of some species inside individual classes. Mass spectra with high mass accuracy and resolving power are acquired using ESI in both positive- and negative-ion modes for the identification of individual species. The composition of esterified fatty acids and polar head groups are characterized based on their characteristic fragment ions obtained from data independent analysis with high and low collision energy. IM is used as an additional separation dimension enabling the separation of lipid classes together with the separation of individual lipid species differing in acyl chain lengths and the number of double bonds coeluting in SFC. Developed SFC/IM-MS method is applied for the comprehensive analysis of lipid composition

Název v anglickém jazyce

Lipidomics: Supercritical fluid chromatography/ion mobility - mass spectrometry as a tool for fast nontargeted analysis of lipids

Popis výsledku anglicky

Individual parameters of SFC analysis have been carefully optimized to achieve a maximum number of separated lipid classes. Developed SFC method enables the fast separation of 16 nonpolar and polar lipid classes within 6 minute analysis including the partial separation of some species inside individual classes. Mass spectra with high mass accuracy and resolving power are acquired using ESI in both positive- and negative-ion modes for the identification of individual species. The composition of esterified fatty acids and polar head groups are characterized based on their characteristic fragment ions obtained from data independent analysis with high and low collision energy. IM is used as an additional separation dimension enabling the separation of lipid classes together with the separation of individual lipid species differing in acyl chain lengths and the number of double bonds coeluting in SFC. Developed SFC/IM-MS method is applied for the comprehensive analysis of lipid composition

Druh

O - Ostatní výsledky

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/LL1302" target="_blank" >LL1302: Hmotnostní spektrometrie při hledání lipidových biomarkerů pro včasnou diagnostiku rakoviny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů