Development of Comprehensive LC/MS Approach for Clinical Lipidomic Applications and Biomarker Discovery
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39899344" target="_blank" >RIV/00216275:25310/14:39899344 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Development of Comprehensive LC/MS Approach for Clinical Lipidomic Applications and Biomarker Discovery
Popis výsledku v původním jazyce
Lipidomics is used in clinical studies to improve the understanding how lipids function in biological systems and for the elucidation of the mechanism of lipid-related diseases. We are developing a comprehensive lipidomic approach based on the combination of several liquid chromatography - mass spectrometry (LC/MS) based methods. Total lipid extracts are separated into individual lipid classes using hydrophilic interaction liquid chromatography (HILIC) [1] or supercritical fluid chromatography (SFC) andquantified by response factor approach for lipid classes [2]. HILIC-LC/MS method enables the separation of multiple polar lipid classes (such as different classes of phospholipids, sphingolipids and glycolipids), but unfortunately nonpolar lipid classescoelute in the void volume of the system, so another normal-phase LC/MS method has been developed for the quantitation of tri-, di- and monoacylglycerols, cholesterol esters and cholesterol. SFC/MS is enables the separation of both polar
Název v anglickém jazyce
Development of Comprehensive LC/MS Approach for Clinical Lipidomic Applications and Biomarker Discovery
Popis výsledku anglicky
Lipidomics is used in clinical studies to improve the understanding how lipids function in biological systems and for the elucidation of the mechanism of lipid-related diseases. We are developing a comprehensive lipidomic approach based on the combination of several liquid chromatography - mass spectrometry (LC/MS) based methods. Total lipid extracts are separated into individual lipid classes using hydrophilic interaction liquid chromatography (HILIC) [1] or supercritical fluid chromatography (SFC) andquantified by response factor approach for lipid classes [2]. HILIC-LC/MS method enables the separation of multiple polar lipid classes (such as different classes of phospholipids, sphingolipids and glycolipids), but unfortunately nonpolar lipid classescoelute in the void volume of the system, so another normal-phase LC/MS method has been developed for the quantitation of tri-, di- and monoacylglycerols, cholesterol esters and cholesterol. SFC/MS is enables the separation of both polar
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/LL1302" target="_blank" >LL1302: Hmotnostní spektrometrie při hledání lipidových biomarkerů pro včasnou diagnostiku rakoviny</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů