Comparison of SFC/MS and UHPLC/MS techniques in the lipidomic characterization of biological samples
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39899343" target="_blank" >RIV/00216275:25310/14:39899343 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Comparison of SFC/MS and UHPLC/MS techniques in the lipidomic characterization of biological samples
Popis výsledku v původním jazyce
Nowadays, supercritical fluid chromatography (SFC) has a renaissance in analytical chemistry due to the commercial availability of reliable SFC systems with potential applications for fast separations of compounds with low to medium polarity, such as lipids. SFC / mass spectrometry (SFC/MS) coupling brings some advantages over conventional (U)HPLC/MS systems, especially in terms of reduced analysis time and therefore higher throughput in the lipidomic analysis. The basic approach for the lipidomic quantitation is based on the direct infusion (shotgun) without any chromatography, but this technique could suffer from suppression effects and the lack of structural details, such as the differentiation of isobaric species. (U)HPLC/MS can be used either forlipid class or lipid species separation. Lipid class separation is achieved by hydrophilic-liquid chromatography (HILIC) for polar lipids or normal-phase (NP) HPLC for nonpolar lipids. Reversed-phase (RP) HPLC enables the resolution of li
Název v anglickém jazyce
Comparison of SFC/MS and UHPLC/MS techniques in the lipidomic characterization of biological samples
Popis výsledku anglicky
Nowadays, supercritical fluid chromatography (SFC) has a renaissance in analytical chemistry due to the commercial availability of reliable SFC systems with potential applications for fast separations of compounds with low to medium polarity, such as lipids. SFC / mass spectrometry (SFC/MS) coupling brings some advantages over conventional (U)HPLC/MS systems, especially in terms of reduced analysis time and therefore higher throughput in the lipidomic analysis. The basic approach for the lipidomic quantitation is based on the direct infusion (shotgun) without any chromatography, but this technique could suffer from suppression effects and the lack of structural details, such as the differentiation of isobaric species. (U)HPLC/MS can be used either forlipid class or lipid species separation. Lipid class separation is achieved by hydrophilic-liquid chromatography (HILIC) for polar lipids or normal-phase (NP) HPLC for nonpolar lipids. Reversed-phase (RP) HPLC enables the resolution of li
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/LL1302" target="_blank" >LL1302: Hmotnostní spektrometrie při hledání lipidových biomarkerů pro včasnou diagnostiku rakoviny</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů