Gradient Elution in Aqueous Normal-Phase Liquid Chromatography on Hydrosilated Silica-Based Stationary Phases

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896310" target="_blank" >RIV/00216275:25310/13:39896310 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S002196731300366X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S002196731300366X</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2013.02.059" target="_blank" >10.1016/j.chroma.2013.02.059</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Gradient Elution in Aqueous Normal-Phase Liquid Chromatography on Hydrosilated Silica-Based Stationary Phases

Popis výsledku v původním jazyce

The possibility of applying a theoretical model in the prediction of the retention of phenolic acids on hydrosilated silica, in aqueous normal phase mode was studied. The actual gradient of the aqueous component in acetonitrile may fluctuate from the pre-set program, as even the gradient-grade acetonitrile contains some water. Hence, the actual concentration of water during the gradient run is higher than pre-set by the gradient program, which leads to lower than expected sample retention. Furthermore,the actual gradient profile may be affected by an increase in water uptake on a polar column during the gradient run. These effects were investigated using the using frontal analysis method and Karl-Fischer titration, for the determination of water in the initial mobile phase, and in the column effluent. Preferential adsorption of water on the Silica hydride, Diamond hydride, UDC Cholesterol, Bidentate C-18, and Phenyl hydride columns can be described by Langmuir isotherms. At the column

Název v anglickém jazyce

Gradient Elution in Aqueous Normal-Phase Liquid Chromatography on Hydrosilated Silica-Based Stationary Phases

Popis výsledku anglicky

The possibility of applying a theoretical model in the prediction of the retention of phenolic acids on hydrosilated silica, in aqueous normal phase mode was studied. The actual gradient of the aqueous component in acetonitrile may fluctuate from the pre-set program, as even the gradient-grade acetonitrile contains some water. Hence, the actual concentration of water during the gradient run is higher than pre-set by the gradient program, which leads to lower than expected sample retention. Furthermore,the actual gradient profile may be affected by an increase in water uptake on a polar column during the gradient run. These effects were investigated using the using frontal analysis method and Karl-Fischer titration, for the determination of water in the initial mobile phase, and in the column effluent. Preferential adsorption of water on the Silica hydride, Diamond hydride, UDC Cholesterol, Bidentate C-18, and Phenyl hydride columns can be described by Langmuir isotherms. At the column

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GAP206%2F12%2F0398" target="_blank" >GAP206/12/0398: Dvourozměrné separace v kapalných médiích</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1286

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

111-118

Kód UT WoS článku

000317156100013

EID výsledku v databázi Scopus

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