Comparison of Nonaqueous Hydrophilic Interaction Chromatography with Aqueous Normal-Phase Chromatography on Hydrosilated Silica-Based Stationary Phases

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896390" target="_blank" >RIV/00216275:25310/13:39896390 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300566/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300566/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201300566" target="_blank" >10.1002/jssc.201300566</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of Nonaqueous Hydrophilic Interaction Chromatography with Aqueous Normal-Phase Chromatography on Hydrosilated Silica-Based Stationary Phases

Popis výsledku v původním jazyce

We investigated the retention behavior of phenolic acids in nonaqueous normal-phase (NP) LC with buffered methanol/acetonitrile mobile phases on hydrosilated silica-based stationary phases. The silica hydride, Diamond hydride, Bidentate C-18, and Cholesterol columns showed a higher retention of phenolic acids in the nonaqueous mobile phases than in aqueous NP mobile phases. There are some selectivity differences between the aqueous and nonaqueous mobile phases, but generally the resolution and selectivity are better in the aqueous systems. The retention of the phenolic acids tested decreased with increasing concentration of methanol in the mobile phase, up to 20% v/v methanol. At increased temperatures, the retention factors and peak widths decrease inboth NP modes, showing linear ln k versus 1/T plots, due to a single retention mechanism over the temperature range from 25 degrees C up to the column stability limit, however, the best separations are achieved at low temperatures. The e

Název v anglickém jazyce

Comparison of Nonaqueous Hydrophilic Interaction Chromatography with Aqueous Normal-Phase Chromatography on Hydrosilated Silica-Based Stationary Phases

Popis výsledku anglicky

We investigated the retention behavior of phenolic acids in nonaqueous normal-phase (NP) LC with buffered methanol/acetonitrile mobile phases on hydrosilated silica-based stationary phases. The silica hydride, Diamond hydride, Bidentate C-18, and Cholesterol columns showed a higher retention of phenolic acids in the nonaqueous mobile phases than in aqueous NP mobile phases. There are some selectivity differences between the aqueous and nonaqueous mobile phases, but generally the resolution and selectivity are better in the aqueous systems. The retention of the phenolic acids tested decreased with increasing concentration of methanol in the mobile phase, up to 20% v/v methanol. At increased temperatures, the retention factors and peak widths decrease inboth NP modes, showing linear ln k versus 1/T plots, due to a single retention mechanism over the temperature range from 25 degrees C up to the column stability limit, however, the best separations are achieved at low temperatures. The e

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GAP206%2F12%2F0398" target="_blank" >GAP206/12/0398: Dvourozměrné separace v kapalných médiích</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

36

Číslo periodika v rámci svazku

17

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

2753-2759

Kód UT WoS článku

000323891300006

EID výsledku v databázi Scopus

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