Highly Stable Surface Modification of Hypercrosslinked Monolithic Capillary Columns and their Application in Hydrophilic Interaction Chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896391" target="_blank" >RIV/00216275:25310/13:39896391 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300395/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300395/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201300395" target="_blank" >10.1002/jssc.201300395</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Highly Stable Surface Modification of Hypercrosslinked Monolithic Capillary Columns and their Application in Hydrophilic Interaction Chromatography

Popis výsledku v původním jazyce

A two-step surface modification of poly(styrene-co-vinylbenzyl chloride-co-divinylbenzene) monolithic stationary phases, including hypercrosslinking and thermally initiated surface grafting of [2-(methacryloyloxy)ethyl]dimethyl(3-sulfopropyl)ammonium hydroxide, has been used to prepare capillary columns for the isocratic separation of small polar compounds in hydrophilic interaction chromatography (HILIC). The prepared monolithic columns provided a dual retention mechanism, combining RP and hydrophilicinteraction chromatography, controlled by the composition of the mobile phase. By the optimization of the surface grafting reaction using response surface methodology, we have found that the grafting time is the main synergistic effect controlling the retention of polar compounds in HILIC. The prepared monolithic columns achieved long column lifetimes and did not lose their separation power following }10 000 injections. Finally, hypercrosslinked columns have been used in the 1- and 2D LC

Název v anglickém jazyce

Highly Stable Surface Modification of Hypercrosslinked Monolithic Capillary Columns and their Application in Hydrophilic Interaction Chromatography

Popis výsledku anglicky

A two-step surface modification of poly(styrene-co-vinylbenzyl chloride-co-divinylbenzene) monolithic stationary phases, including hypercrosslinking and thermally initiated surface grafting of [2-(methacryloyloxy)ethyl]dimethyl(3-sulfopropyl)ammonium hydroxide, has been used to prepare capillary columns for the isocratic separation of small polar compounds in hydrophilic interaction chromatography (HILIC). The prepared monolithic columns provided a dual retention mechanism, combining RP and hydrophilicinteraction chromatography, controlled by the composition of the mobile phase. By the optimization of the surface grafting reaction using response surface methodology, we have found that the grafting time is the main synergistic effect controlling the retention of polar compounds in HILIC. The prepared monolithic columns achieved long column lifetimes and did not lose their separation power following }10 000 injections. Finally, hypercrosslinked columns have been used in the 1- and 2D LC

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GPP206%2F12%2FP049" target="_blank" >GPP206/12/P049: Příprava a modifikace vysoce zesítěných monolitických stacionárních fází pro separace malých molekul v kapilární kapalinové chromatografii</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

36

Číslo periodika v rámci svazku

17

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

2806-2812

Kód UT WoS článku

000323891300011

EID výsledku v databázi Scopus

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