The cytotoxic effect of alfa-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896772" target="_blank" >RIV/00216275:25310/13:39896772 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.3892/or.2013.2778" target="_blank" >http://dx.doi.org/10.3892/or.2013.2778</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/or.2013.2778" target="_blank" >10.3892/or.2013.2778</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The cytotoxic effect of alfa-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction

Popis výsledku v původním jazyce

In recent years, alfa-tomatine has been studied for its anticancer activity. In the present study, we focused on the cytotoxic effect of alfa-tomatine in the MCF-7 human breast adenocarcinoma cell line, its mechanism of action, biotransformation and stability in the culture medium. We observed an inhibition of cell proliferation and viability at concentrations of 6 and 9 mikroM but then a recovery of cells occurred. The recovery was not caused by the biotransformation of alfa-tomatine in MCF-7 cells, but by a substantial decrease in the concentration of alfa-tomatine in the culture medium due to its binding with cholesterol. Regarding the mechanism of action of alfa-tomatine, we observed no DNA damage, no changes in the levels of the proteins p53 and p21(WAF1/Cip1), and no apoptosis (neither activated caspase-8 and -9, nor sub-G1 peak, or morphological signs). We found a loss of ATP in alfa-tomatine-treated cells. These results support the conclusion that alfa-tomatine does not induce

Název v anglickém jazyce

The cytotoxic effect of alfa-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction

Popis výsledku anglicky

In recent years, alfa-tomatine has been studied for its anticancer activity. In the present study, we focused on the cytotoxic effect of alfa-tomatine in the MCF-7 human breast adenocarcinoma cell line, its mechanism of action, biotransformation and stability in the culture medium. We observed an inhibition of cell proliferation and viability at concentrations of 6 and 9 mikroM but then a recovery of cells occurred. The recovery was not caused by the biotransformation of alfa-tomatine in MCF-7 cells, but by a substantial decrease in the concentration of alfa-tomatine in the culture medium due to its binding with cholesterol. Regarding the mechanism of action of alfa-tomatine, we observed no DNA damage, no changes in the levels of the proteins p53 and p21(WAF1/Cip1), and no apoptosis (neither activated caspase-8 and -9, nor sub-G1 peak, or morphological signs). We found a loss of ATP in alfa-tomatine-treated cells. These results support the conclusion that alfa-tomatine does not induce

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Oncology Reports

ISSN

1021-335X

e-ISSN

—

Svazek periodika

30

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

10

Strana od-do

2593-2602

Kód UT WoS článku

000330226200007

EID výsledku v databázi Scopus

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